Effects of Cytokines on Clonal Expansion of CD34+/c-kit+/CD13+Cells at 2 Weeks
| . | MC . | MC/Monocyte . | Monocyte . | Other . |
|---|---|---|---|---|
| IL-3, SCF, IL-6 | 11/480 | 10/480 | 2/480 | 0/480 |
| IL-3, SCF, IL-6, TPO, Epo | 18/480 | 16/480 | 2/480 | 0/480 |
| IL-3, SCF, IL-6, Epo, GM-CSF, IGF-1, bFGF | 16/480 | 12/480 | 6/480 | 0/480 |
| . | MC . | MC/Monocyte . | Monocyte . | Other . |
|---|---|---|---|---|
| IL-3, SCF, IL-6 | 11/480 | 10/480 | 2/480 | 0/480 |
| IL-3, SCF, IL-6, TPO, Epo | 18/480 | 16/480 | 2/480 | 0/480 |
| IL-3, SCF, IL-6, Epo, GM-CSF, IGF-1, bFGF | 16/480 | 12/480 | 6/480 | 0/480 |
Cells were individually deposited per well in 96-well plates containing serum-free media with designated cytokines. Incubations were performed for 14 days at 37°C in a humidified atmosphere of 5% CO2/95% air. Each well was scored positive for proliferation when ≥5 cells were identified. Data represent the number of positive wells/total number of wells seeded.
Abbreviations: MC, mast cells; Other, includes erythrocytes, myeloid cells, lymphocytes, and megakaryocytes.