Growth of Mast Cells From Single- and Double-Sorted CD34+/c-kit+/CD13+(+/+) and CD34+/c-kit+/CD13−(+/−) Cells
First Sort | First Sort | |||||||
+/+ | +/− | |||||||
Week | Tot | MC | M | O | Tot | MC | M | O |
×106 | ×106 | |||||||
4 | 1.82 | 1.50 | 0.32 | 0 | 5.00 | 0.25 | 4.65 | 0.10 |
Second Sort | Second Sort | |||||||
+/+ | +/− | |||||||
Week | Tot | MC | M | O | Tot | MC | M | O |
×106 | ×106 | |||||||
4 | 1.21 | 1.00 | 0.21 | 0 | 3.80 | 0.07 | 3.61 | 0.12 |
First Sort | First Sort | |||||||
+/+ | +/− | |||||||
Week | Tot | MC | M | O | Tot | MC | M | O |
×106 | ×106 | |||||||
4 | 1.82 | 1.50 | 0.32 | 0 | 5.00 | 0.25 | 4.65 | 0.10 |
Second Sort | Second Sort | |||||||
+/+ | +/− | |||||||
Week | Tot | MC | M | O | Tot | MC | M | O |
×106 | ×106 | |||||||
4 | 1.21 | 1.00 | 0.21 | 0 | 3.80 | 0.07 | 3.61 | 0.12 |
Flasks were initially seeded with 1.5 × 105 cells (50,000 cells/mL). CD34+/c-kit+/CD13−cells recovered after first sort were resorted into CD34+/c-kit+/CD13+(+/+) and CD34+/c-kit+/CD13−(+/−) populations and cultured.
Abbreviations: Tot, total cells; MC, mast cells; M, monocytes; O, other cells.