Table 2.

Growth of Mast Cells From Single- and Double-Sorted CD34+/c-kit+/CD13+(+/+) and CD34+/c-kit+/CD13(+/−) Cells

 First Sort  First Sort  
 +/+ +/−  
Week  Tot MC Tot MC O  
 ×106 ×106 
4  1.82  1.50  0.32 0  5.00  0.25  4.65  0.10 
 Second Sort  Second Sort  
 +/+ +/−  
Week  Tot MC Tot MC O  
 ×106 ×106 
4  1.21  1.00  0.21 0  3.80  0.07  3.61  0.12 
 First Sort  First Sort  
 +/+ +/−  
Week  Tot MC Tot MC O  
 ×106 ×106 
4  1.82  1.50  0.32 0  5.00  0.25  4.65  0.10 
 Second Sort  Second Sort  
 +/+ +/−  
Week  Tot MC Tot MC O  
 ×106 ×106 
4  1.21  1.00  0.21 0  3.80  0.07  3.61  0.12 

Flasks were initially seeded with 1.5 × 105 cells (50,000 cells/mL). CD34+/c-kit+/CD13cells recovered after first sort were resorted into CD34+/c-kit+/CD13+(+/+) and CD34+/c-kit+/CD13(+/−) populations and cultured.

Abbreviations: Tot, total cells; MC, mast cells; M, monocytes; O, other cells.

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