Effects of Calreticulin and Calreticulin Fragments on Angiogenesis In Vivo
| Additions to Matrigel . | Mean Surface Area Occupied by Cells (mm2/ 1.26 × 105 mm2) . | Inhibition (%) . |
|---|---|---|
| 1. None | 671 | |
| bFGF | 17,732 | |
| bFGF + GST-calreticulin (5 μg/mL) | 4,616 | 74 |
| bFGF + GST-calreticulin (2.5 μg/mL) | 6,387 | 64 |
| bFGF + GST-calreticulin (1.25 μg/mL) | 9,870 | 44 |
| 2. None | 487 | |
| bFGF | 14,472 | |
| bFGF + MBP-calreticulin (5 μg/mL) | 5,112 | 65 |
| bFGF + MBP-vasostatin (5 μg/mL) | 4,989 | 66 |
| 3. None | 1,329 | |
| bFGF | 8,320 | |
| bFGF + cleaved calreticulin (5 μg/mL) | 3,464 | 58 |
| bFGF + MBP–Δ120-calreticulin (15 μg/mL) | 4,291 | 48 |
| bFGF + MBP 120-180 calreticulin (5 μg/mL) | 2,458 | 70 |
| Additions to Matrigel . | Mean Surface Area Occupied by Cells (mm2/ 1.26 × 105 mm2) . | Inhibition (%) . |
|---|---|---|
| 1. None | 671 | |
| bFGF | 17,732 | |
| bFGF + GST-calreticulin (5 μg/mL) | 4,616 | 74 |
| bFGF + GST-calreticulin (2.5 μg/mL) | 6,387 | 64 |
| bFGF + GST-calreticulin (1.25 μg/mL) | 9,870 | 44 |
| 2. None | 487 | |
| bFGF | 14,472 | |
| bFGF + MBP-calreticulin (5 μg/mL) | 5,112 | 65 |
| bFGF + MBP-vasostatin (5 μg/mL) | 4,989 | 66 |
| 3. None | 1,329 | |
| bFGF | 8,320 | |
| bFGF + cleaved calreticulin (5 μg/mL) | 3,464 | 58 |
| bFGF + MBP–Δ120-calreticulin (15 μg/mL) | 4,291 | 48 |
| bFGF + MBP 120-180 calreticulin (5 μg/mL) | 2,458 | 70 |
Mice were injected sc with Matrigel alone, Matrigel plus bFGF (150 ng/mL), Matrigel plus bFGF (150 ng/mL) plus GST-calreticulin, MBP-calreticulin, MBP-vasostatin, calreticulin cleaved and purified from MBP-calreticulin, MBP–Δ120-calreticulin, MBP-calreticulin 120-180 peptide, or MBP. Plugs were removed after 5 to 7 days, and histological sections were stained with Masson’s trichrome. The results reflect the mean surface area (expressed in mm2) occupied by cells within a circular surface area of 1.26 × 105 mm2; 12 to 15 nonoverlapping fields were scanned in each plug; there were 5 plugs/group. Determinations of surface area were performed by a semi-automated digitalized analyzer.