Progenitor Cell Distribution by hCD4 Level invav-hCD4 Bone Marrow
| Cells Cultured* . | Stimulus† . | No. of Colonies . | Progenitors in Fraction‡ . | |||||
|---|---|---|---|---|---|---|---|---|
| Blast . | G . | GM . | M . | Eo . | Meg . | |||
| Unstained | SCF | 31 | 47 | 6 | 5 | 0 | 0 | — |
| IL-3 + TPO | 16 | 59 | 14 | 18 | 1 | 37 | — | |
| M-CSF | 0 | 9 | 11 | 51 | 0 | 0 | — | |
| Unsorted | SCF | 24 | 47 | 3 | 5 | 0 | 0 | — |
| IL-3 + TPO | 21 | 54 | 20 | 11 | 2 | 32 | — | |
| M-CSF | 0 | 13 | 11 | 52 | 0 | 0 | — | |
| Low-negative | SCF | 0 | 0 | 0 | 0 | 0 | 0 | 0% |
| IL-3 + TPO | 0 | 2 | 0 | 0 | 0 | 0 | 0% | |
| M-CSF | 0 | 0 | 0 | 0 | 0 | 0 | 0% | |
| Intermediate | SCF | 0 | 3 | 0 | 0 | 0 | 0 | 2% |
| IL-3 + TPO | 0 | 8 | 1 | 6 | 5 | 2 | 8% | |
| M-CSF | 0 | 0 | 3 | 7 | 0 | 0 | 7% | |
| High | SCF | 48 | 73 | 8 | 0 | 0 | 0 | 98% |
| IL-3 + TPO | 25 | 68 | 18 | 30 | 5 | 53 | 92% | |
| M-CSF | 0 | 10 | 18 | 83 | 0 | 0 | 93% | |
| Cells Cultured* . | Stimulus† . | No. of Colonies . | Progenitors in Fraction‡ . | |||||
|---|---|---|---|---|---|---|---|---|
| Blast . | G . | GM . | M . | Eo . | Meg . | |||
| Unstained | SCF | 31 | 47 | 6 | 5 | 0 | 0 | — |
| IL-3 + TPO | 16 | 59 | 14 | 18 | 1 | 37 | — | |
| M-CSF | 0 | 9 | 11 | 51 | 0 | 0 | — | |
| Unsorted | SCF | 24 | 47 | 3 | 5 | 0 | 0 | — |
| IL-3 + TPO | 21 | 54 | 20 | 11 | 2 | 32 | — | |
| M-CSF | 0 | 13 | 11 | 52 | 0 | 0 | — | |
| Low-negative | SCF | 0 | 0 | 0 | 0 | 0 | 0 | 0% |
| IL-3 + TPO | 0 | 2 | 0 | 0 | 0 | 0 | 0% | |
| M-CSF | 0 | 0 | 0 | 0 | 0 | 0 | 0% | |
| Intermediate | SCF | 0 | 3 | 0 | 0 | 0 | 0 | 2% |
| IL-3 + TPO | 0 | 8 | 1 | 6 | 5 | 2 | 8% | |
| M-CSF | 0 | 0 | 3 | 7 | 0 | 0 | 7% | |
| High | SCF | 48 | 73 | 8 | 0 | 0 | 0 | 98% |
| IL-3 + TPO | 25 | 68 | 18 | 30 | 5 | 53 | 92% | |
| M-CSF | 0 | 10 | 18 | 83 | 0 | 0 | 93% | |
Abbreviations: Blast, blast cell; G, granulocyte; GM, granulocyte-macrophage; M, macrophage; Eo, eosinophil; Meg, megakaryocyte.
All cultures were initiated with 25,000 nucleated bone marrow cells from HS21/45 vav-hCD4 mice (10 to 13 weeks old). Data shown are from a representative fractionation experiment (1 of 3), namely that shown in Fig 4.
The recombinant murine factors were as follows: stem cell factor (SCF; 100 ng/mL); IL-3 (10 ng/mL); thrombopoietin (TPO; 50 ng/mL); and macrophage colony-stimulating factor (M-CSF; 10 ng/mL).
The progenitors (colony-forming cells) in each fraction, as a percentage of the progenitors recovered in the 3 fractions, adjusted for the different proportions of total nucleated cells in the 3 fractions (see Fig 4 legend).