Table 3. Structural Features of TCR... | American Society of Hematology

Table 3.

Structural Features of TCR β-Chain Transcripts Expressed by FHL Clone(s)

Patient No.	V β Gene	J β Gene	Junctional Sequence			6 Subclones Examined^3-150
Patient No.	V β Gene	J β Gene	V β	ND β N	J β	6 Subclones Examined^3-150
1	13.6	1.2	C^3-151 ASS	TSAL	TTGTT	5
			TGT GCC AGC AGT	TAC TCG AGA CTG	ACC TAT GGC TAC ACC
2	5.1	1.6	C ASS	PAGGA	SPLH	5
			TGC GCC AGC AGC	TTC GCC GGT GGG AAT	TCA CCC CTC CAC
3	4.1	1.4	CSV	TGVP	MLACP	5
			TGC AGC GTT	TGG GGG GTT CCG	ATG AAA AAC TGT TTT
4	6.4	1.1	CASS	PPGGLA	TGAP	3
			TGT GCC AGC AGC	CCC TTC GAG GGA TTG AAC	ACT GAA GCT TTC
	6.4	1.1	CAS	AATGPG	GAP	2
			TGT GCC AGC	AGG GAC ACG GGT TTT GAG	CAA GCT TTC
5	6.5	1.1	CASS	AAAV	TGAP	5
			TGT GCC AGC AGC	CGG GAC AGG GTT	ACT GAA GCT TTC

Patient No.	V β Gene	J β Gene	Junctional Sequence			6 Subclones Examined^3-150
Patient No.	V β Gene	J β Gene	V β	ND β N	J β	6 Subclones Examined^3-150
1	13.6	1.2	C^3-151 ASS	TSAL	TTGTT	5
			TGT GCC AGC AGT	TAC TCG AGA CTG	ACC TAT GGC TAC ACC
2	5.1	1.6	C ASS	PAGGA	SPLH	5
			TGC GCC AGC AGC	TTC GCC GGT GGG AAT	TCA CCC CTC CAC
3	4.1	1.4	CSV	TGVP	MLACP	5
			TGC AGC GTT	TGG GGG GTT CCG	ATG AAA AAC TGT TTT
4	6.4	1.1	CASS	PPGGLA	TGAP	3
			TGT GCC AGC AGC	CCC TTC GAG GGA TTG AAC	ACT GAA GCT TTC
	6.4	1.1	CAS	AATGPG	GAP	2
			TGT GCC AGC	AGG GAC ACG GGT TTT GAG	CAA GCT TTC
5	6.5	1.1	CASS	AAAV	TGAP	5
			TGT GCC AGC AGC	CGG GAC AGG GTT	ACT GAA GCT TTC

The underline indicates a part of Dβ gene segment.

The Vβ-Jβ fragments (Fig 7) were subcloned into plasmids and sequenced. Six individual colonies were analyzed.

Amino acid residues represented by single-letter abbreviations. D β 1.1, GGGACAGGGGGC; D β 2.1, GGGACTAGCGGGAGGG.30