Coexpression of CD Antigens on 97A6+Cells
| . | PB CD34− . | PB CD34+ . | BM CD34− . | BM CD34+ . | PB CML . | d3 PB (+IL-3) . | d14 PB (+IL-3) . |
|---|---|---|---|---|---|---|---|
| CD10 | − | nd | − | − | − | nd | − |
| CD13 | + | − | ± | ± | ± | − | ± |
| CD16 | − | nd | nd | nd | ± | nd | − |
| CD17 | + | − | ± | ± | ± | ± | − |
| CD26 | + | − | + | ± | + | ± | ± |
| CD33 | ± | + | + | + | + | + | + |
| CD38 | + | + | + | + | + | + | ± |
| CD44 | + | + | + | + | + | + | ± |
| CD55 | + | + | + | + | + | + | + |
| CD59 | + | + | + | + | + | + | + |
| CD71 | − | + | ± | + | − | + | ± |
| CD81 | ± | nd | + | + | + | + | + |
| CD117 | − | + | − | + | − | + | nd |
| CD123 | + | + | + | + | + | ± | nd |
| CD164 | + | + | + | + | + | − | ± |
| IgE | + | ± | + | + | − | − | ± |
| HLA-DR | − | ± | ± | + | − | + | ± |
| . | PB CD34− . | PB CD34+ . | BM CD34− . | BM CD34+ . | PB CML . | d3 PB (+IL-3) . | d14 PB (+IL-3) . |
|---|---|---|---|---|---|---|---|
| CD10 | − | nd | − | − | − | nd | − |
| CD13 | + | − | ± | ± | ± | − | ± |
| CD16 | − | nd | nd | nd | ± | nd | − |
| CD17 | + | − | ± | ± | ± | ± | − |
| CD26 | + | − | + | ± | + | ± | ± |
| CD33 | ± | + | + | + | + | + | + |
| CD38 | + | + | + | + | + | + | ± |
| CD44 | + | + | + | + | + | + | ± |
| CD55 | + | + | + | + | + | + | + |
| CD59 | + | + | + | + | + | + | + |
| CD71 | − | + | ± | + | − | + | ± |
| CD81 | ± | nd | + | + | + | + | + |
| CD117 | − | + | − | + | − | + | nd |
| CD123 | + | + | + | + | + | ± | nd |
| CD164 | + | + | + | + | + | − | ± |
| IgE | + | ± | + | + | − | − | ± |
| HLA-DR | − | ± | ± | + | − | + | ± |
BM and PB cells were labeled with anti-CD34-PerCP, 97A6-PE, and FITC-conjugated antibodies of the indicated specificities and analyzed on a FACSCalibur flow cytometer. CD34− and CD34+ cells were evaluated after gating on the respective populations using the Cellquest software. CML PB cells and CD34+ PB cells stimulated with 100 ng/mL IL-3 and harvested at days 3 and 14, respectively, were stained with 97A6-PE and FITC-conjugated antibodies of the indicated specificities. ± indicates positive subpopulation.
Abbreviation: nd, not determined.