Correlations Between Percentages of GPI−CFU-GEMM in Donor Bone Marrow and GPI− Granulocytes in the Donor and Recipient Blood
| Experiment . | % GPI Negative Colonies in CFU-GEMM* . | % (±SD) GPI Negative Granulocytes in . | No. of Recipients . | |
|---|---|---|---|---|
| Donor Blood† . | Recipient Blood† . | |||
| 1 | 38 | 49.0 | 34.2 (±11.2) | 6 |
| 2 | 47 | 40.4 | 35.2 (±7.2) | 5 |
| 3 | 74 | 45.0 | 66.6 (±0.8) | 5 |
| 4‡ | 82 | 81.4 | 85.4 (±1.5) | 3 |
| 5 | 60 | 51.5 | 59.7 (±4.6) | 3 |
| 6‡ | 100 | 89.7 | 97.9 (±0.3) | 3 |
| Experiment . | % GPI Negative Colonies in CFU-GEMM* . | % (±SD) GPI Negative Granulocytes in . | No. of Recipients . | |
|---|---|---|---|---|
| Donor Blood† . | Recipient Blood† . | |||
| 1 | 38 | 49.0 | 34.2 (±11.2) | 6 |
| 2 | 47 | 40.4 | 35.2 (±7.2) | 5 |
| 3 | 74 | 45.0 | 66.6 (±0.8) | 5 |
| 4‡ | 82 | 81.4 | 85.4 (±1.5) | 3 |
| 5 | 60 | 51.5 | 59.7 (±4.6) | 3 |
| 6‡ | 100 | 89.7 | 97.9 (±0.3) | 3 |
Abbreviation: SD, standard deviation.
In each experiment, bone marrow cells from a mouse transplanted with mosaic fetal liver cells were cultured in vitro to assess the percentage of GPI− CFU-GEMM. The same bone marrow cells were transplanted to lethally irradiated secondary recipients.
Blood granulocytes from the donors of bone marrow and the recipients were analyzed with FACS to determine the percentage of GPI− cells.
A mouse that received 5 × 105 mosaic fetal liver cells and had a high percentage of GPI− granulocytes was used as a donor for the second transplantation.