Irradiated Allogeneic Splenocytes Do Not Produce GVHD When Transplanted Into MHC Mismatched Recipients
| . | BM Alone . | BM + Irradiated Splenocytes . | BM + Nonirradiated Splenocytes . |
|---|---|---|---|
| Mean weight (g) | 35 ± 1.4* | 35 ± 1.8* | 24.4 ± 3.4 |
| Mean spleen weight (mg) | 160 ± 30* | 150 ± 30* | 30 ± 20 |
| Mean histologic score for liver GVHD | 0.8 ± 0.4* | 0.1 ± 0.4* | 2.7 ± 0.5 |
| Mean histologic score for overall GVHD | 2 ± 0.7† | 0.7 ± 1* | 4.3 ± 1 |
| n = | 5 | 7 | 7 |
| . | BM Alone . | BM + Irradiated Splenocytes . | BM + Nonirradiated Splenocytes . |
|---|---|---|---|
| Mean weight (g) | 35 ± 1.4* | 35 ± 1.8* | 24.4 ± 3.4 |
| Mean spleen weight (mg) | 160 ± 30* | 150 ± 30* | 30 ± 20 |
| Mean histologic score for liver GVHD | 0.8 ± 0.4* | 0.1 ± 0.4* | 2.7 ± 0.5 |
| Mean histologic score for overall GVHD | 2 ± 0.7† | 0.7 ± 1* | 4.3 ± 1 |
| n = | 5 | 7 | 7 |
F1 mice (C57.BL6 × SJL) were irradiated to 10 Gy in 2 equal fractions and then transplanted with 1 × 106 SJL TCD BM cells on day 0. Two additional groups of mice received an equal number of BM plus 30 × 106 irradiated (7.5 Gy) SJL splenocytes or 30 × 106 nonirradiated SJL splenocytes on day 0. One mouse each died in the groups receiving BM alone or BM plus nonirradiated splenocytes before day +5. On day +51, all surviving mice were euthanized, weighed, and subjected to necropsy. Hematoxylin-eosin–stained sections of formalin fixed, paraffin embedded skin, intestinal tract epithelium, and liver were examined microscopically by an observer (JH) unaware of the treatment group. The mean body weight, spleen weight, and mean scores for the liver GVHD and overall GVHD for each treatment group are shown.
Differences between mean values compared with recipients of nonirradiated splenocytes are significant at a level of P < .0001.
Differences between mean values compared with recipients of nonirradiated splenocytes are significant at a level of P < .002.