Donor T Cells Mediate the Graft Facilitating Activity of Irradiated Allogeneic Splenocytes
| . | BM + Irrad Spleen . | BM + TCD Irrad Spleen . | BM Alone . | . | . |
|---|---|---|---|---|---|
| Fraction surviving | 11/18 | 7/10 | 18/18 | ||
| Fraction donor engrafted | 9/11 | 2/7 | 6/18 | ||
| PValues | |||||
| Irrad Sp v TCD Irrad Sp | Irrad Sp v BM | ||||
| % Donor cells | 100 ± 42 | 5 ± 48 | 0 ± 48 | P = .042 | P = .025 |
| T cells | |||||
| % Donor BM | 100 ± 42 | 13 ± 32 | 0 ± 47 | P = .021 | P = .023 |
| % Donor spleen | 0 ± 10 | 0 ± 0 | 0 ± 1 | ||
| % Host | 0 ± 40 | 88 ± 36 | 100 ± 47 | P = .030 | P = .018 |
| . | BM + Irrad Spleen . | BM + TCD Irrad Spleen . | BM Alone . | . | . |
|---|---|---|---|---|---|
| Fraction surviving | 11/18 | 7/10 | 18/18 | ||
| Fraction donor engrafted | 9/11 | 2/7 | 6/18 | ||
| PValues | |||||
| Irrad Sp v TCD Irrad Sp | Irrad Sp v BM | ||||
| % Donor cells | 100 ± 42 | 5 ± 48 | 0 ± 48 | P = .042 | P = .025 |
| T cells | |||||
| % Donor BM | 100 ± 42 | 13 ± 32 | 0 ± 47 | P = .021 | P = .023 |
| % Donor spleen | 0 ± 10 | 0 ± 0 | 0 ± 1 | ||
| % Host | 0 ± 40 | 88 ± 36 | 100 ± 47 | P = .030 | P = .018 |
B10.BR mice received 11 Gy irradiation on day −2 and were then transplanted with 1 × 106 BM cells from C57.BL6 mice, or a combination of BM and 75 × 106 irradiated (7.5 Gy) C57.BL6 splenocytes or BM and 60 × 106 TCD irradiated (7.5 Gy) C57.BL6 splenocytes. The C57.BL6 donor mice were all CD45.1+; BM and spleen donors varied according to their expression of Thy 1.1 (spleen) or Thy 1.2 (BM). The median percentages of peripheral blood cells that were donor-derived (CD45.1+) and the percentage of blood T cells that were derived from donor BM, donor spleen, or were of host origin were determined at day +60 to day +70 by flow cytometry.