Concentration of Different Lineage Positive (Lin+) Cells in the Sca-1+ and Sca-1+Lin− Cell Fractions
| Experiment . | Cell Fraction . | Lineage Positive (%) . | |||
|---|---|---|---|---|---|
| CD45/B220 . | CD11b . | CD8 . | CD4 . | ||
| 1 | Unseparated | 39.6 | 12.7 | 0.6 | 1.2 |
| Sca-1+ | 66.5 | 1.4 | 2.7 | 9.5 | |
| Sca-1+Lin− | 4.4 | 0 | <0.1 | 0 | |
| 2 | Unseparated | 50.7 | 7.0 | 0.7 | 1.7 |
| Sca-1+ | 67.6 | 4.0 | 3.4 | 10.6 | |
| Sca-1+Lin− | 3.8 | 0.7 | <0.2 | 0 | |
| Experiment . | Cell Fraction . | Lineage Positive (%) . | |||
|---|---|---|---|---|---|
| CD45/B220 . | CD11b . | CD8 . | CD4 . | ||
| 1 | Unseparated | 39.6 | 12.7 | 0.6 | 1.2 |
| Sca-1+ | 66.5 | 1.4 | 2.7 | 9.5 | |
| Sca-1+Lin− | 4.4 | 0 | <0.1 | 0 | |
| 2 | Unseparated | 50.7 | 7.0 | 0.7 | 1.7 |
| Sca-1+ | 67.6 | 4.0 | 3.4 | 10.6 | |
| Sca-1+Lin− | 3.8 | 0.7 | <0.2 | 0 | |
BM cells were subjected to a stem cell purification procedure based on their expression of stem cell antigen-1 (Sca-1) and the lack of expression of cell surface antigens associated with differentiated hematopoietic cell lineages typical for B cells (CD45/B220), myelomonocytic cells (CD11b/mac-1), and T cells (CD4/L3T4, CD8/Ly-2), using MACS Sca-1 Multisort kit (see Materials and Methods). The fractions were analyzed by flow cytometry. The results represent 2 experiments in which the purity of Sca-1+Lin− was 56.6% and 63.4%, compared with 1.7% and 1.4% in the unseparated fraction and 4.2% and 5.0% in the Sca-1+ cell fraction.