Precursor Expansion and Phenotype of Thymocytes Generated in FTOC From Nef− and Nef+Transduced ISP4+ Precursors
| . | Weeks of Culture . | |||||
|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | ||||
| Nef− . | Nef+ . | Nef− . | Nef+ . | Nef− . | Nef+ . | |
| Precursor expansion* | 10.0 ± 4.0 | 2.5 ± 0.8 | 12.6 ± 0.8 | 1.7 ± 1.4 | 2.5 ± 1.3 | 0.2 ± 0.2 |
| Phenotype† | ||||||
| CD4+CD8β+ | 93.2 ± 1.2 | 14.4 ± 3.0 | 71.1 ± 1.9 | 5.2 ± 0.6 | 76.1 ± 4.1 | 4 ± 5.2 |
| CD4+CD8βdim/− | 4.0 ± 1.0 | 3.5 ± 2.4 | 22.1 ± 3.8 | 5.2 ± 2.5 | 9.0 ± 3.3 | 2 ± 3.2 |
| CD4dim/−CD8β+ | 1.1 ± 0.4 | 65.1 ± 7.3 | 3.0 ± 2.3 | 48.6 ± 15.1 | 9.1 ± 6.0 | 40 ± 18.4 |
| CD4dim/−CD8βdim/− | 1.7 ± 0.6 | 17.0 ± 2.9 | 3.8 ± 0.5 | 41 ± 13.2 | 5.8 ± 1.4 | 54 ± 26.9 |
| CD3+CD4+ | 64.0 ± 2.8 | 18.2 ± 5.5 | 53.5 ± 8.0 | 9.7 ± 1.3 | 55.0 ± 6.6 | 6 ± 4.5 |
| CD3+CD4dim | 0.9 ± 0.6 | 34.0 ± 4.2 | 9.8 ± 0.4 | 40.4 ± 11.2 | 11.4 ± 0.6 | 26 ± 10.9 |
| CD3+CD4− | 0.9 ± 0.2 | 18.4 ± 7.2 | 2.3 ± 0.4 | 23.8 ± 1.3 | 2.5 ± 0.2 | 49 ± 29.0 |
| CD3+CD8β+/dim | 61.9 ± 1.1 | 61.2 ± 1.3 | ND | ND | 56.1 ± 7.0 | 42 ± 3.9 |
| CD3+CD8β− | 1.3 ± 0.6 | 5.2 ± 1.1 | ND | ND | 8.3 ± 0.5 | 41 ± 6.4 |
| . | Weeks of Culture . | |||||
|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | ||||
| Nef− . | Nef+ . | Nef− . | Nef+ . | Nef− . | Nef+ . | |
| Precursor expansion* | 10.0 ± 4.0 | 2.5 ± 0.8 | 12.6 ± 0.8 | 1.7 ± 1.4 | 2.5 ± 1.3 | 0.2 ± 0.2 |
| Phenotype† | ||||||
| CD4+CD8β+ | 93.2 ± 1.2 | 14.4 ± 3.0 | 71.1 ± 1.9 | 5.2 ± 0.6 | 76.1 ± 4.1 | 4 ± 5.2 |
| CD4+CD8βdim/− | 4.0 ± 1.0 | 3.5 ± 2.4 | 22.1 ± 3.8 | 5.2 ± 2.5 | 9.0 ± 3.3 | 2 ± 3.2 |
| CD4dim/−CD8β+ | 1.1 ± 0.4 | 65.1 ± 7.3 | 3.0 ± 2.3 | 48.6 ± 15.1 | 9.1 ± 6.0 | 40 ± 18.4 |
| CD4dim/−CD8βdim/− | 1.7 ± 0.6 | 17.0 ± 2.9 | 3.8 ± 0.5 | 41 ± 13.2 | 5.8 ± 1.4 | 54 ± 26.9 |
| CD3+CD4+ | 64.0 ± 2.8 | 18.2 ± 5.5 | 53.5 ± 8.0 | 9.7 ± 1.3 | 55.0 ± 6.6 | 6 ± 4.5 |
| CD3+CD4dim | 0.9 ± 0.6 | 34.0 ± 4.2 | 9.8 ± 0.4 | 40.4 ± 11.2 | 11.4 ± 0.6 | 26 ± 10.9 |
| CD3+CD4− | 0.9 ± 0.2 | 18.4 ± 7.2 | 2.3 ± 0.4 | 23.8 ± 1.3 | 2.5 ± 0.2 | 49 ± 29.0 |
| CD3+CD8β+/dim | 61.9 ± 1.1 | 61.2 ± 1.3 | ND | ND | 56.1 ± 7.0 | 42 ± 3.9 |
| CD3+CD8β− | 1.3 ± 0.6 | 5.2 ± 1.1 | ND | ND | 8.3 ± 0.5 | 41 ± 6.4 |
Figures indicate average on experiments with 3 different donors +/− standard deviation.
Abbreviation: ND, not determined.
Precursor expansion: ratio of output thymocyte number to input progenitor number.
Phenotype, percentage of cells of corresponding phenotype on total thymocyte population. For CD4 and CD8 β, +, expression above level delimited by quadstat as in Fig 3D; −, expression below level delimited by quadstat as in Fig 3B; dim, expression level between − and +. For CD3, + and −, expression above or below level delimited by quadstat as in Fig 3B.