Summary of Events Mediated by c-mpl and Its Mutants in UT-7
| . | TPO . | GM-CSF . | |||
|---|---|---|---|---|---|
| UT- mplwt . | UT- mplΔ7 . | UT- mplΔ7ΔC . | UT- mplΔ8 . | UT- mplΔ7* . | |
| Proliferation | ++++ | +++ | − | − | ++++ |
| Differentiation | ++++ | − | − | − | − |
| Jak-2 | ++++ | − | − | − | ++ |
| Tyk-2 | ++++ | − | − | − | ND |
| STATs | ++++ | − | ND | ND | ND |
| Tec | ++ | ++ | ND | ND | ND |
| Shc | ++++ | + | − | − | +++ |
| Vav | ++++ | + | − | − | ++ |
| c-mpl | ++++ | − | − | − | ND |
| MAPK | ++++ | + | − | − | ++ |
| Akt | ++++ | +/− | − | − | ++ |
| PI 3-kinase | ++++ | + | ND | ND | ++++ |
| . | TPO . | GM-CSF . | |||
|---|---|---|---|---|---|
| UT- mplwt . | UT- mplΔ7 . | UT- mplΔ7ΔC . | UT- mplΔ8 . | UT- mplΔ7* . | |
| Proliferation | ++++ | +++ | − | − | ++++ |
| Differentiation | ++++ | − | − | − | − |
| Jak-2 | ++++ | − | − | − | ++ |
| Tyk-2 | ++++ | − | − | − | ND |
| STATs | ++++ | − | ND | ND | ND |
| Tec | ++ | ++ | ND | ND | ND |
| Shc | ++++ | + | − | − | +++ |
| Vav | ++++ | + | − | − | ++ |
| c-mpl | ++++ | − | − | − | ND |
| MAPK | ++++ | + | − | − | ++ |
| Akt | ++++ | +/− | − | − | ++ |
| PI 3-kinase | ++++ | + | ND | ND | ++++ |
Abbreviation: ND, not determined.
Identical results were obtained when UT-mplwt cells were stimulated with GM-CSF. The GM-CSF–stimulated phosphorylation of Jak-2, Vav, MAPK, and Akt in both UT-mplwt and UT-mplΔ7 cells was weaker than the TPO-stimulated phosphorylation in UT-mplwt.