Reduced Cytoplasmic Expression of IFN-γ and TNF- by Activated UCB T Cells
Cord Blood . | CB01 . | CB02 . | CB03 . | CB04 . | Adult Blood . | AB01 . | AB02 . | AB03 . | AB04 . |
---|---|---|---|---|---|---|---|---|---|
Activated T cells expressing cytoplasmic IFN-γ (%) | |||||||||
Unstim. | 0.0 | 0.0 | 0.0 | 0.0 | Unstim. | 0.0 | 0.0 | 0.0 | 0.0 |
18 h | 0.0 | 0.0 | 3.8 | 2.1 | 18 h | 2.8 | 1.1 | 4.3 | 5.7 |
40 h | 0.0 | 0.0 | 2.9 | 2.9 | 40 h | 1.2 | 1.0 | 5.1 | ND |
Activated T cells expressing cytoplasmic TNF-α (%) | |||||||||
Unstim. | 0.0 | 0.0 | 0.0 | 0.0 | Unstim. | 0.0 | 0.0 | 0.0 | 0.0 |
18 h | 0.4 | 0.3 | 5.5 | 4.7 | 18 h | 6.4 | 4.5 | 4.5 | 7.9 |
40 h | 4.8 | 8.3 | 5.0 | 7.1 | 40 h | 13.0 | 10.0 | 9.3 | ND |
Cord Blood . | CB01 . | CB02 . | CB03 . | CB04 . | Adult Blood . | AB01 . | AB02 . | AB03 . | AB04 . |
---|---|---|---|---|---|---|---|---|---|
Activated T cells expressing cytoplasmic IFN-γ (%) | |||||||||
Unstim. | 0.0 | 0.0 | 0.0 | 0.0 | Unstim. | 0.0 | 0.0 | 0.0 | 0.0 |
18 h | 0.0 | 0.0 | 3.8 | 2.1 | 18 h | 2.8 | 1.1 | 4.3 | 5.7 |
40 h | 0.0 | 0.0 | 2.9 | 2.9 | 40 h | 1.2 | 1.0 | 5.1 | ND |
Activated T cells expressing cytoplasmic TNF-α (%) | |||||||||
Unstim. | 0.0 | 0.0 | 0.0 | 0.0 | Unstim. | 0.0 | 0.0 | 0.0 | 0.0 |
18 h | 0.4 | 0.3 | 5.5 | 4.7 | 18 h | 6.4 | 4.5 | 4.5 | 7.9 |
40 h | 4.8 | 8.3 | 5.0 | 7.1 | 40 h | 13.0 | 10.0 | 9.3 | ND |
Abbreviations: h, hours; ND, not determined.