Table 1.

Reduced Cytoplasmic Expression of IFN-γ and TNF- by Activated UCB T Cells

Cord Blood CB01 CB02 CB03 CB04 Adult BloodAB01 AB02 AB03 AB04
Activated T cells expressing cytoplasmic IFN-γ (%)
 
Unstim.  0.0  0.0 0.0  0.0  Unstim.  0.0  0.0  0.0  0.0  
18 h 0.0  0.0  3.8  2.1  18 h  2.8  1.1  4.3 5.7  
40 h  0.0  0.0  2.9  2.9  40 h 1.2  1.0  5.1  ND  
Activated T cells expressing cytoplasmic TNF-α (%)
 
Unstim.  0.0  0.0 0.0  0.0  Unstim.  0.0  0.0  0.0  0.0  
18 h 0.4  0.3  5.5  4.7  18 h  6.4  4.5  4.5 7.9  
40 h  4.8  8.3  5.0  7.1  40 h  13.0 10.0  9.3  ND 
Cord Blood CB01 CB02 CB03 CB04 Adult BloodAB01 AB02 AB03 AB04
Activated T cells expressing cytoplasmic IFN-γ (%)
 
Unstim.  0.0  0.0 0.0  0.0  Unstim.  0.0  0.0  0.0  0.0  
18 h 0.0  0.0  3.8  2.1  18 h  2.8  1.1  4.3 5.7  
40 h  0.0  0.0  2.9  2.9  40 h 1.2  1.0  5.1  ND  
Activated T cells expressing cytoplasmic TNF-α (%)
 
Unstim.  0.0  0.0 0.0  0.0  Unstim.  0.0  0.0  0.0  0.0  
18 h 0.4  0.3  5.5  4.7  18 h  6.4  4.5  4.5 7.9  
40 h  4.8  8.3  5.0  7.1  40 h  13.0 10.0  9.3  ND 

Abbreviations: h, hours; ND, not determined.

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