Expression and Function of CXCR4 on Cell Lines Representing Different Stages of B-Cell Development
| B-cell Lines . | Percentage of Cells Expressing CXCR4 . | Chemotaxis Toward SDF-1 . | Calcium Mobilization After SDF-1 Stimulation . |
|---|---|---|---|
| Pro-B cells | |||
| RS 4-11 | 71.5 | ++ | − |
| Nalm 16 | 32.25 | ++ | + |
| Jim-1 | 99.8 | + | − |
| REH | 99.5 | + | + |
| Pre-B cells | |||
| 697 | 60.17 | ++ | + |
| Nalm-6 | 99.1 | ++ | + |
| Blin-1 | 98.9 | ND | ND |
| Mature B cells | |||
| 20A | 0.89 | − | − |
| 15A | 22.73 | − | − |
| RS11846 | 17.6 | + | − |
| HS-Sultan | 23.9 | + | − |
| SU-DHL-6 | 52.04 | + | ND |
| B-cell Lines . | Percentage of Cells Expressing CXCR4 . | Chemotaxis Toward SDF-1 . | Calcium Mobilization After SDF-1 Stimulation . |
|---|---|---|---|
| Pro-B cells | |||
| RS 4-11 | 71.5 | ++ | − |
| Nalm 16 | 32.25 | ++ | + |
| Jim-1 | 99.8 | + | − |
| REH | 99.5 | + | + |
| Pre-B cells | |||
| 697 | 60.17 | ++ | + |
| Nalm-6 | 99.1 | ++ | + |
| Blin-1 | 98.9 | ND | ND |
| Mature B cells | |||
| 20A | 0.89 | − | − |
| 15A | 22.73 | − | − |
| RS11846 | 17.6 | + | − |
| HS-Sultan | 23.9 | + | − |
| SU-DHL-6 | 52.04 | + | ND |
In the column representing chemotaxis toward SDF-1, (−) denotes a migration index less than 2, (+) denotes a migration index between 2 and 7, and (++) denotes a migration index more than 7. In the column representing calcium mobilization After SDF-1 Stimulation, (−) represents lack of detectable calcium mobilization and (+) represents the presence of detectable calcium mobilization.
Abbreviation: ND, not determined.