Proliferation Assay of PEL Cells
| Reagents . | cpm/Culture . | ||
|---|---|---|---|
| BC-1 . | BCP-1 . | BCBL-1 . | |
| Experiment no. 1* | |||
| VEGF/VPF 50 ng/mL | 20,804 ± 1,088 | 4,738 ± 413 | 21,875 ± 853 |
| VEGF/VPF 10 ng/mL | 20,206 ± 953 | 4,970 ± 316 | 21,651 ± 702 |
| VEGF/VPF 2 ng/mL | 20,220 ± 1,101 | 5,469 ± 199 | 22,706 ± 975 |
| Anti-VEGF/VPF Ab (10 μg/mL)† | 20,732 ± 883 | 5,319 ± 298 | 22,624 ± 756 |
| Medium alone | 20,870 ± 643 | 5,199 ± 185 | 22,737 ± 1,324 |
| Experiment no. 2‡ | |||
| Anti-VEGF/VPF Ab (10 μg/ml)† | 42,809 ± 3,474 | 12,764 ± 415 | 35,387 ± 2,371 |
| Medium alone | 38,057 ± 2,977 | 14,144 ± 1,164 | 37,383 ± 1,471 |
| Reagents . | cpm/Culture . | ||
|---|---|---|---|
| BC-1 . | BCP-1 . | BCBL-1 . | |
| Experiment no. 1* | |||
| VEGF/VPF 50 ng/mL | 20,804 ± 1,088 | 4,738 ± 413 | 21,875 ± 853 |
| VEGF/VPF 10 ng/mL | 20,206 ± 953 | 4,970 ± 316 | 21,651 ± 702 |
| VEGF/VPF 2 ng/mL | 20,220 ± 1,101 | 5,469 ± 199 | 22,706 ± 975 |
| Anti-VEGF/VPF Ab (10 μg/mL)† | 20,732 ± 883 | 5,319 ± 298 | 22,624 ± 756 |
| Medium alone | 20,870 ± 643 | 5,199 ± 185 | 22,737 ± 1,324 |
| Experiment no. 2‡ | |||
| Anti-VEGF/VPF Ab (10 μg/ml)† | 42,809 ± 3,474 | 12,764 ± 415 | 35,387 ± 2,371 |
| Medium alone | 38,057 ± 2,977 | 14,144 ± 1,164 | 37,383 ± 1,471 |
PEL cells (1 × 104 cells/well) were cultured for 48 hours, including a final 6-hour pulse with [3H]-thymidine. The assay was performed in triplicate. Data show the mean (±SD) of 2 independent experiments.
Assay was performed in PRMI1640 plus 1% FBS.
Conditioned media prepared as described in Materials and Methods were used in these experiments. The concentration of FBS in each conditioned media was adjusted to 1% or 10% before incubating with antihuman VEGF/VPF Ab for 1 hour at room temperature. Cells were then cultured for 48 hours as described above.
Assay was performed in PRMI1640 plus 10% FBS.