Transduction Efficiency of Cultured MKs After Adenoviral Infection
| . | % GFP-Positive MKs . | ||
|---|---|---|---|
| 24 h . | 48 h . | 72 h . | |
| Experiment no. 1 | |||
| MOI 10 | 6% | 12% | |
| MOI 20 | 9% | 17% | |
| MOI 100 | 11% | 23% | |
| MOI 200 | 24% | 48% | |
| Experiment no. 2 | |||
| MOI 100 | 6% | 17% | 29% |
| MOI 200 | 10% | 27% | 46% |
| . | % GFP-Positive MKs . | ||
|---|---|---|---|
| 24 h . | 48 h . | 72 h . | |
| Experiment no. 1 | |||
| MOI 10 | 6% | 12% | |
| MOI 20 | 9% | 17% | |
| MOI 100 | 11% | 23% | |
| MOI 200 | 24% | 48% | |
| Experiment no. 2 | |||
| MOI 100 | 6% | 17% | 29% |
| MOI 200 | 10% | 27% | 46% |
CD34+ cells were cultured with PEG-rHuMGDF for 11 days before coincubation with AdCMVhGFP, virus control, or vehicle control at the indicated MOI. Cells were harvested at the indicated time points and incubated with PE-GPIb antibody before cytometric acquisition of data. Live cells were gated and MKs defined by PE fluorescence and forward scatter as described in Materials and Methods. FITC fluorescence histograms were generated from the MK population. FITC fluorescence positivity gates were set at 1% for no virus control. hGFP positivity was ≤2% for all adenovirus control samples.