Binding of IRF Complexes to Mutated ISG15 ISRE Oligonucleotides
| Oligo . | Sequence . | Binding* . | ||||
|---|---|---|---|---|---|---|
| B Lymphocyte . | Macrophage . | |||||
| ICSBP . | IRF-2 . | IRF-4 . | ICSBP . | IRF-4 . | ||
| ISRE | GATCCTCGGGAAAGGGAAACCGAAACTGAAGCC | ++ | ++ | ++ | ++ | ++ |
| M1 | GATCCTCGGGAAAGGGcgtCCGAAACTGAAGCC | − | − | − | − | − |
| M2 | GATCCTCGGGcgtGGGcgtCCGAAACTGAAGCC | − | − | − | − | − |
| M3 | GATCCTCGGGcgtGGGcgtCCGcgtCTGAAGCC | − | − | − | − | − |
| M4 | GATCCTCGGGcgtGGGAAACCGAAACTGAAGCC | + | + | + | + | + |
| M5 | GATCCTCGGGAAAGGGAAACCGcgtCTGAAGCC | − | − | − | − | − |
| GG1 | GATCCTCGGGAAAGGggGAAACCGAAACTGAAGCC | + | + | − | + | + |
| GG2 | GATCCTCGGGAAAGGGAAACCggGAAACTGAAGCC | − | − | − | − | − |
| Oligo . | Sequence . | Binding* . | ||||
|---|---|---|---|---|---|---|
| B Lymphocyte . | Macrophage . | |||||
| ICSBP . | IRF-2 . | IRF-4 . | ICSBP . | IRF-4 . | ||
| ISRE | GATCCTCGGGAAAGGGAAACCGAAACTGAAGCC | ++ | ++ | ++ | ++ | ++ |
| M1 | GATCCTCGGGAAAGGGcgtCCGAAACTGAAGCC | − | − | − | − | − |
| M2 | GATCCTCGGGcgtGGGcgtCCGAAACTGAAGCC | − | − | − | − | − |
| M3 | GATCCTCGGGcgtGGGcgtCCGcgtCTGAAGCC | − | − | − | − | − |
| M4 | GATCCTCGGGcgtGGGAAACCGAAACTGAAGCC | + | + | + | + | + |
| M5 | GATCCTCGGGAAAGGGAAACCGcgtCTGAAGCC | − | − | − | − | − |
| GG1 | GATCCTCGGGAAAGGggGAAACCGAAACTGAAGCC | + | + | − | + | + |
| GG2 | GATCCTCGGGAAAGGGAAACCggGAAACTGAAGCC | − | − | − | − | − |
Abbreviations: ++, strong binding; +, weak binding; −, no binding.
The presence of individual IRF proteins was concluded from positive supershifts with the corresponding antibody. The mobility of protein complexes formed with mutated oligonucleotides is in part altered.