Flow Cytometric Analysis of 7 Different Rh SDM Mutant K562 Lines Using Monoclonal Anti-D
| Anti- EpD1-9 . | Anti-EpD1-37 . | ISBT No. . | K562/ pBabe . | K562/ cE . | K562/ D . | K562/ lp3 . | K562/ lp3 + 6 . | K562/ lp4 . | K562/ lp4 + 6 . | K562/ lp3 + 4 . | K562/ lp3 + 4 + 6 . | K562/ lp6 . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EpD1 | EpD1 GAD 2 | — | 3.2 | 3.6 | 9.1 | 3.9 | 2.9 | 3.5 | 3.4 | 4.1 | 4.6 | 3.1 |
| EpD1 | EpD1 Reg A | — | 3.3 | 3.4 | 9.3 | 3.6 | 2.8 | 3.3 | 3.4 | 3.9 | 3.8 | 2.9 |
| EpD1 | EpD1 LHM169/81 | 82 | 4.2 | 4.1 | 17.1 | 4.1 | 3.2 | 3.6 | 5.3 | 4.2 | 7.8 | 3.6 |
| EpD2 | EpD3 D6D-02 | 123 | 3.5 | 3.7 | 13.3 | 3.2 | 3.1 | 3.3 | 3.6 | 3.9 | 6.3 | 3.3 |
| EpD2 | EpD3 L87 1G7 | 108 | 4.1 | 4.2 | 22.9 | 4.2 | 3.4 | 3.7 | 6.7 | 4.1 | 24.1 | 4.2 |
| EpD2 | EpD3 P3X249 | 5.7 | 6.1 | 24.4 | 5.2 | 5.6 | 4.9 | 6.8 | 7.1 | 19.1 | 5.1 | |
| EpD3 | EpD5 LOR29 FF749 | 49 | 3.9 | 4.2 | 20.1 | 4.4 | 5.6 | 3.7 | 6.7 | 4.1 | 23.3 | 3.7 |
| EpD3 | EpD5 LHM 76/55 | 75 | 4.3 | 4.3 | 29.4 | 4.3 | 5.9 | 4.0 | 30.8 | 4.2 | 32.3 | 3.4 |
| EpD3 | EpD5 LHM 76/59 | 76 | 5.3 | 5.9 | 25.3 | 5.4 | 27.7 | 5.0 | 51.7 | 5.0 | 27.0 | 14.9 |
| EpD3 | EpD5 H41-11B7 | 106 | 4.4 | 4.2 | 23.3 | 3.9 | 35.2 | 3.9 | 31.1 | 4.2 | 31.1 | 32.7 |
| EpD3 | EpD5/35 P3X290 | 4.1 | 4.2 | 21.6 | 4.2 | 3.7 | 3.5 | 5.2 | 4.3 | 22.2 | 3.4 | |
| EpD4 | EpD6 LOR 176C7 | 45 | 3.4 | 3.7 | 6.6 | 3.8 | 3.1 | 3.2 | 8.0 | 4.1 | 10.5 | 3.6 |
| EpD5 | EpD7 CAZ 7 4C5 | 42 | 3.8 | 3.5 | 8.6 | 2.3 | 6.0 | 2.6 | 6.1 | 3.8 | 10.1 | 2.5 |
| EpD5 | EpD8 D10 | 114 | 5.7 | 5.6 | 35.7 | 5.3 | 3.7 | 4.6 | 6.8 | 5.0 | 21.7 | 3.5 |
| EpD5 | EpD10 BS229 | 109 | 4.1 | 4.2 | 21.2 | 4.0 | 3.4 | 3.4 | 3.9 | 7.1 | 12.9 | 3.4 |
| EpD5 | EpD10 MAR1/F8 | 52 | 4.5 | 4.9 | 22.1 | 3.6 | 2.9 | 5.4 | 5.6 | 15.5 | 35.1 | 5.2 |
| EpD5 | EpD10 BS231 | 110 | 3.4 | 3.8 | 13.1 | 3.6 | 3.1 | 3.2 | 3.6 | 4.9 | 6.1 | 3.3 |
| EpD5 | EpD11 HAM-A | 3.8 | 4.1 | 17.1 | 2.4 | 2.8 | 3.2 | 3.0 | 20.2 | 25.0 | 2.6 | |
| EpD6/7 | EpD12 LOR178D3 | 46 | 4.7 | 4.5 | 27.1 | 4.6 | 3.5 | 4.3 | 5.3 | 25.2 | 33.5 | 3.6 |
| EpD6/7 | EpD12 P3AF6 | 102 | 5.2 | 6.5 | 30.9 | 6.5 | 6.6 | 5.1 | 7.8 | 26.2 | 30.6 | 5.2 |
| EpD6/7 | EpD12 175-2 | 35 | 3.4 | 3.0 | 13.4 | 2.8 | 6.2 | 5.7 | 24.4 | 15.8 | 17.5 | 2.3 |
| EpD6/7 | EpD12 MS201 | 113 | 7.2 | 10.0 | 26.9 | 10.1 | 13.5 | 11.9 | 45.3 | 32.9 | 35.6 | 18.3* |
| EpD6/7 | EpD12 P3X61 | ND | 8.3 | 45.4 | 5.3 | 13.1 | 11.7 | 12.7 | 66.1 | 58.9 | 8.4 | |
| EpD6/7 | EpD13 F5S | 90 | 6.5 | 6.4 | 23.9 | 6.1 | 4.5 | 3.9 | 4.8 | 22.0 | 18.4 | 4.2 |
| EpD6/7 | EpD13 BRAD3 | 105 | ND | 4.1 | 21.4 | 2.6 | 6.7 | 2.9 | 3.3 | 7.5 | 21.9 | 3.0 |
| EpD6/7 | EpD15 BTSN4 | 71 | 5.8 | 4.9 | 32.9 | 4.7 | 7.3 | 4.6 | 41.0 | 12.5* | 28.9 | 3.3 |
| EpD6/7 | EpD15 D-90/7 | 31 | 5.5 | 5.1 | 35.2 | 5.5 | 3.8 | 4.5 | 5.2 | 4.7 | 20.6 | 3.4 |
| EpD6/7 | EpD15 L87 1G7 | 4.2 | 22.9 | 4.2 | 3.4 | 3.7 | 5.7 | 4.1 | 24.1 | 4.2 | ||
| EpD6/7 | EpD17 NaTh28-C11 | 37 | 3.5 | 4.4 | 22.0 | 5.9 | 6.1 | 3.5 | 9.4 | 22.9 | 29.2 | 4.3 |
| EpD6/7 | EpD17 P3 187 | 98 | 3.0 | 3.3 | 18.8 | 4.1 | 4.7 | 9.9 | 30.6 | 22.6 | 21.2 | 3.8 |
| EpD6/7 | EpD18 HG/92 (G) | 30 | 5.3 | 4.3 | 36.0 | 4.6 | 3.5 | 4.2 | 4.9 | 4.3 | 19.0 | 3.1 |
| EpD6/7 | EpD18 T3A2F6 (G) | ND | 6.4 | 32.6 | 3.5 | 11.6 | 6.4 | 2.4 | 54.5 | 50.7 | 7.5 | |
| EpD6/7 | EpD18 HM10 (M) | ND | 10.2 | 45.0 | 8.5 | 16.6 | 8.9 | 10.0 | 58.2 | 65.2 | 7.6 | |
| EpD6/7 | EpD19 MAD2 (M) | ND | 5.1 | 20.2 | 3.1 | 3.6 | 3.4 | 3.9 | 47.5 | 51.6 | 3.1 | |
| EpD6/7 | EpD21 HIRO-2 | 122 | 6.7 | 7.6 | 20.2 | 6.4 | 6.6 | 5.0 | 5.1 | 16.5 | 18.9 | 7.4 |
| EpD8 | EpD22 LHM76/58 | 74 | 4.8 | 5.2 | 26.5 | 4.4 | 3.4 | 3.7 | 4.4 | 5.9 | 5.9 | 3.6 |
| EpD8 | EpD22 LHM59/19 | 78 | 4.4 | 4.3 | 25.5 | 4.3 | 3.5 | 3.9 | 4.4 | 5.4 | 6.5 | 3.3 |
| EpD9 | EpD23 BIRMA D6 | 96 | 5.0 | 4.6 | 30.4 | 4.2 | 32.7 | 3.9 | 52.4 | 4.2 | 29.0 | 13.1 |
| EpD9 | EpD23 P3G6 | 101 | 4.3 | 4.2 | 26.6 | 3.9 | 5.0 | 3.5 | 15.8 | 4.0 | 20.4 | 2.9 |
| EpD9 | EpD23 HIRO-4 | 118 | 3.8 | 3.8 | 15.6 | 3.7 | 3.5 | 3.4 | 5.2 | 3.8 | 11.3 | 3.2 |
| EpD9 | EpD23 MS26 | 112 | 5.7 | 6.3 | 27.0 | 3.7 | 18.7 | 4.6 | 20.2 | 4.7 | 32.7 | 12.6 |
| EpD9 | EpD23 P3X21223B | 5.8 | 6.9 | 27.6 | 3.2 | 56.3 | 4.7 | 37.3 | 4.3 | 33.4 | 58.5 | |
| EpD30 8D8 | 4.6 | 4.9 | 25.3 | 5.8 | 9.3 | 5.4 | 40.8 | 4.9 | 25.7 | 8.4 | ||
| EpD32 ZIG189 | 62 | ND | 5.9 | 14.1 | 3.1 | 4.2 | 3.5 | 3.6 | 7.8 | 5.0 | 3.6 | |
| EpD34 LORA/F | 50 | 4.7 | 5.4 | 14.2 | 5.7 | 3.8 | 4.7 | 5.1 | 5.1 | 11.1 | 4.1 | |
| EpD35 SALSA | 60 | 5.1 | 7.8 | 28.1 | 7.7 | 4.5 | 5.6 | 6.1 | 6.1 | 10.3* | 6.1 | |
| EpD36 NAV3 2E8 | 54 | 4.7 | 5.7 | 26.0 | 6.7 | 5.9 | 6.0 | 8.5 | 5.6 | 24.0 | 7.6 | |
| EpD36 NAV6/F 4D5 | 55 | 5.2 | 7.4 | 31.7 | 6.8 | 6.4 | 6.1 | 30.4 | 5.8 | 26.5 | 10.8* | |
| EpD37 BTSN10 | 73 | 5.7 | 5.6 | 32.7 | 6.4 | 4.2 | 6.6 | 11.3* | 5.9 | 11.9* | 8.6 | |
| EpD37 MCAD6 | 124 | 5.8 | 6.3 | 33.2 | 6.6 | 5.9 | 5.4 | 7.9 | 11.7* | 32.7 | 4.8 |
| Anti- EpD1-9 . | Anti-EpD1-37 . | ISBT No. . | K562/ pBabe . | K562/ cE . | K562/ D . | K562/ lp3 . | K562/ lp3 + 6 . | K562/ lp4 . | K562/ lp4 + 6 . | K562/ lp3 + 4 . | K562/ lp3 + 4 + 6 . | K562/ lp6 . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EpD1 | EpD1 GAD 2 | — | 3.2 | 3.6 | 9.1 | 3.9 | 2.9 | 3.5 | 3.4 | 4.1 | 4.6 | 3.1 |
| EpD1 | EpD1 Reg A | — | 3.3 | 3.4 | 9.3 | 3.6 | 2.8 | 3.3 | 3.4 | 3.9 | 3.8 | 2.9 |
| EpD1 | EpD1 LHM169/81 | 82 | 4.2 | 4.1 | 17.1 | 4.1 | 3.2 | 3.6 | 5.3 | 4.2 | 7.8 | 3.6 |
| EpD2 | EpD3 D6D-02 | 123 | 3.5 | 3.7 | 13.3 | 3.2 | 3.1 | 3.3 | 3.6 | 3.9 | 6.3 | 3.3 |
| EpD2 | EpD3 L87 1G7 | 108 | 4.1 | 4.2 | 22.9 | 4.2 | 3.4 | 3.7 | 6.7 | 4.1 | 24.1 | 4.2 |
| EpD2 | EpD3 P3X249 | 5.7 | 6.1 | 24.4 | 5.2 | 5.6 | 4.9 | 6.8 | 7.1 | 19.1 | 5.1 | |
| EpD3 | EpD5 LOR29 FF749 | 49 | 3.9 | 4.2 | 20.1 | 4.4 | 5.6 | 3.7 | 6.7 | 4.1 | 23.3 | 3.7 |
| EpD3 | EpD5 LHM 76/55 | 75 | 4.3 | 4.3 | 29.4 | 4.3 | 5.9 | 4.0 | 30.8 | 4.2 | 32.3 | 3.4 |
| EpD3 | EpD5 LHM 76/59 | 76 | 5.3 | 5.9 | 25.3 | 5.4 | 27.7 | 5.0 | 51.7 | 5.0 | 27.0 | 14.9 |
| EpD3 | EpD5 H41-11B7 | 106 | 4.4 | 4.2 | 23.3 | 3.9 | 35.2 | 3.9 | 31.1 | 4.2 | 31.1 | 32.7 |
| EpD3 | EpD5/35 P3X290 | 4.1 | 4.2 | 21.6 | 4.2 | 3.7 | 3.5 | 5.2 | 4.3 | 22.2 | 3.4 | |
| EpD4 | EpD6 LOR 176C7 | 45 | 3.4 | 3.7 | 6.6 | 3.8 | 3.1 | 3.2 | 8.0 | 4.1 | 10.5 | 3.6 |
| EpD5 | EpD7 CAZ 7 4C5 | 42 | 3.8 | 3.5 | 8.6 | 2.3 | 6.0 | 2.6 | 6.1 | 3.8 | 10.1 | 2.5 |
| EpD5 | EpD8 D10 | 114 | 5.7 | 5.6 | 35.7 | 5.3 | 3.7 | 4.6 | 6.8 | 5.0 | 21.7 | 3.5 |
| EpD5 | EpD10 BS229 | 109 | 4.1 | 4.2 | 21.2 | 4.0 | 3.4 | 3.4 | 3.9 | 7.1 | 12.9 | 3.4 |
| EpD5 | EpD10 MAR1/F8 | 52 | 4.5 | 4.9 | 22.1 | 3.6 | 2.9 | 5.4 | 5.6 | 15.5 | 35.1 | 5.2 |
| EpD5 | EpD10 BS231 | 110 | 3.4 | 3.8 | 13.1 | 3.6 | 3.1 | 3.2 | 3.6 | 4.9 | 6.1 | 3.3 |
| EpD5 | EpD11 HAM-A | 3.8 | 4.1 | 17.1 | 2.4 | 2.8 | 3.2 | 3.0 | 20.2 | 25.0 | 2.6 | |
| EpD6/7 | EpD12 LOR178D3 | 46 | 4.7 | 4.5 | 27.1 | 4.6 | 3.5 | 4.3 | 5.3 | 25.2 | 33.5 | 3.6 |
| EpD6/7 | EpD12 P3AF6 | 102 | 5.2 | 6.5 | 30.9 | 6.5 | 6.6 | 5.1 | 7.8 | 26.2 | 30.6 | 5.2 |
| EpD6/7 | EpD12 175-2 | 35 | 3.4 | 3.0 | 13.4 | 2.8 | 6.2 | 5.7 | 24.4 | 15.8 | 17.5 | 2.3 |
| EpD6/7 | EpD12 MS201 | 113 | 7.2 | 10.0 | 26.9 | 10.1 | 13.5 | 11.9 | 45.3 | 32.9 | 35.6 | 18.3* |
| EpD6/7 | EpD12 P3X61 | ND | 8.3 | 45.4 | 5.3 | 13.1 | 11.7 | 12.7 | 66.1 | 58.9 | 8.4 | |
| EpD6/7 | EpD13 F5S | 90 | 6.5 | 6.4 | 23.9 | 6.1 | 4.5 | 3.9 | 4.8 | 22.0 | 18.4 | 4.2 |
| EpD6/7 | EpD13 BRAD3 | 105 | ND | 4.1 | 21.4 | 2.6 | 6.7 | 2.9 | 3.3 | 7.5 | 21.9 | 3.0 |
| EpD6/7 | EpD15 BTSN4 | 71 | 5.8 | 4.9 | 32.9 | 4.7 | 7.3 | 4.6 | 41.0 | 12.5* | 28.9 | 3.3 |
| EpD6/7 | EpD15 D-90/7 | 31 | 5.5 | 5.1 | 35.2 | 5.5 | 3.8 | 4.5 | 5.2 | 4.7 | 20.6 | 3.4 |
| EpD6/7 | EpD15 L87 1G7 | 4.2 | 22.9 | 4.2 | 3.4 | 3.7 | 5.7 | 4.1 | 24.1 | 4.2 | ||
| EpD6/7 | EpD17 NaTh28-C11 | 37 | 3.5 | 4.4 | 22.0 | 5.9 | 6.1 | 3.5 | 9.4 | 22.9 | 29.2 | 4.3 |
| EpD6/7 | EpD17 P3 187 | 98 | 3.0 | 3.3 | 18.8 | 4.1 | 4.7 | 9.9 | 30.6 | 22.6 | 21.2 | 3.8 |
| EpD6/7 | EpD18 HG/92 (G) | 30 | 5.3 | 4.3 | 36.0 | 4.6 | 3.5 | 4.2 | 4.9 | 4.3 | 19.0 | 3.1 |
| EpD6/7 | EpD18 T3A2F6 (G) | ND | 6.4 | 32.6 | 3.5 | 11.6 | 6.4 | 2.4 | 54.5 | 50.7 | 7.5 | |
| EpD6/7 | EpD18 HM10 (M) | ND | 10.2 | 45.0 | 8.5 | 16.6 | 8.9 | 10.0 | 58.2 | 65.2 | 7.6 | |
| EpD6/7 | EpD19 MAD2 (M) | ND | 5.1 | 20.2 | 3.1 | 3.6 | 3.4 | 3.9 | 47.5 | 51.6 | 3.1 | |
| EpD6/7 | EpD21 HIRO-2 | 122 | 6.7 | 7.6 | 20.2 | 6.4 | 6.6 | 5.0 | 5.1 | 16.5 | 18.9 | 7.4 |
| EpD8 | EpD22 LHM76/58 | 74 | 4.8 | 5.2 | 26.5 | 4.4 | 3.4 | 3.7 | 4.4 | 5.9 | 5.9 | 3.6 |
| EpD8 | EpD22 LHM59/19 | 78 | 4.4 | 4.3 | 25.5 | 4.3 | 3.5 | 3.9 | 4.4 | 5.4 | 6.5 | 3.3 |
| EpD9 | EpD23 BIRMA D6 | 96 | 5.0 | 4.6 | 30.4 | 4.2 | 32.7 | 3.9 | 52.4 | 4.2 | 29.0 | 13.1 |
| EpD9 | EpD23 P3G6 | 101 | 4.3 | 4.2 | 26.6 | 3.9 | 5.0 | 3.5 | 15.8 | 4.0 | 20.4 | 2.9 |
| EpD9 | EpD23 HIRO-4 | 118 | 3.8 | 3.8 | 15.6 | 3.7 | 3.5 | 3.4 | 5.2 | 3.8 | 11.3 | 3.2 |
| EpD9 | EpD23 MS26 | 112 | 5.7 | 6.3 | 27.0 | 3.7 | 18.7 | 4.6 | 20.2 | 4.7 | 32.7 | 12.6 |
| EpD9 | EpD23 P3X21223B | 5.8 | 6.9 | 27.6 | 3.2 | 56.3 | 4.7 | 37.3 | 4.3 | 33.4 | 58.5 | |
| EpD30 8D8 | 4.6 | 4.9 | 25.3 | 5.8 | 9.3 | 5.4 | 40.8 | 4.9 | 25.7 | 8.4 | ||
| EpD32 ZIG189 | 62 | ND | 5.9 | 14.1 | 3.1 | 4.2 | 3.5 | 3.6 | 7.8 | 5.0 | 3.6 | |
| EpD34 LORA/F | 50 | 4.7 | 5.4 | 14.2 | 5.7 | 3.8 | 4.7 | 5.1 | 5.1 | 11.1 | 4.1 | |
| EpD35 SALSA | 60 | 5.1 | 7.8 | 28.1 | 7.7 | 4.5 | 5.6 | 6.1 | 6.1 | 10.3* | 6.1 | |
| EpD36 NAV3 2E8 | 54 | 4.7 | 5.7 | 26.0 | 6.7 | 5.9 | 6.0 | 8.5 | 5.6 | 24.0 | 7.6 | |
| EpD36 NAV6/F 4D5 | 55 | 5.2 | 7.4 | 31.7 | 6.8 | 6.4 | 6.1 | 30.4 | 5.8 | 26.5 | 10.8* | |
| EpD37 BTSN10 | 73 | 5.7 | 5.6 | 32.7 | 6.4 | 4.2 | 6.6 | 11.3* | 5.9 | 11.9* | 8.6 | |
| EpD37 MCAD6 | 124 | 5.8 | 6.3 | 33.2 | 6.6 | 5.9 | 5.4 | 7.9 | 11.7* | 32.7 | 4.8 |
Values are expressed as the mean fluorescence intensity. Values twice that of background antibody binding (ie, that observed binding to a negative control K562 line) are considered positive and are shaded gray and boldfaced. The epitope specificity of each anti-D is shown using either the 1-9 model12,13 or the 1-37 model.14 The identification number allocated to each anti-D in the recent third workshop on MoAbs against red blood cell antigens is also shown in the column labeled ISBT No.
Weakly positive samples.