TCR CDR3 Size Distribution Analysis of Allo-BMT Recipients at 3 and 12 Months Post-BMT in CD4+ and CD8+ T-Cell Subsets
| Recipient/ Sample . | 3 mo Post-BMT . | 12 mo Post-BMT . | ||
|---|---|---|---|---|
| 4/8 Ratio4-150 . | Spectratype4-151 . | 4/8 Ratio . | Spectratype . | |
| I | ||||
| UPN 272 | 0.22 | D/LH | 0.28 | G |
| UPN 279 | 2.60 | D/LH‡ | 2.40 | G |
| II | ||||
| UPN 298 | 0.21 | D/LH | 0.23 | G/LH |
| UPN 301 | 1.7 | LH/G | 1.5 | G |
| III | ||||
| UPN 275 | 0.10 | LH/G | 0.32 | G |
| UPN 285 | 2.10 | LH/G | 3.90 | G |
| Recipient/ Sample . | 3 mo Post-BMT . | 12 mo Post-BMT . | ||
|---|---|---|---|---|
| 4/8 Ratio4-150 . | Spectratype4-151 . | 4/8 Ratio . | Spectratype . | |
| I | ||||
| UPN 272 | 0.22 | D/LH | 0.28 | G |
| UPN 279 | 2.60 | D/LH‡ | 2.40 | G |
| II | ||||
| UPN 298 | 0.21 | D/LH | 0.23 | G/LH |
| UPN 301 | 1.7 | LH/G | 1.5 | G |
| III | ||||
| UPN 275 | 0.10 | LH/G | 0.32 | G |
| UPN 285 | 2.10 | LH/G | 3.90 | G |
CD4/CD8 T-cell ratio was determined after staining PBMC with appropriate MoAbs and FACS sorting; only true CD3+CD8+high T cells were calculated.
Spectratype classification: G = Gaussian, ie, normal distribution of TCR CDR3 sizes with 7 to 12 bands in the majority of the TCRBV families; LH, less heterogenous size distribution, ie, reduced number of bands and/or with different band intensity (3 to 5 dominant bands, 1 to 3 accompanying bands); D, deviated size distribution pattern, severely reduced number of bands (1 to 4 bands) with different band intensity; D/LH, deviated in the majority of the TCRBV families and a less heterogenous distribution in less than 30% of the TCRBV families in CD4+ as well as CD8+ T-cell subsets; LH/G, less heterogenous distribution in the majority of the TCRBV families and a Gaussian distribution in less than 30% of the TCRBV families in both T-cell subsets.
The deviated TCR size distribution patterns were more frequently found in CD8+ than in CD4+ T cells.