TT-Specific Proliferative Responses of T-Cell Lines at Several Time-Points After Allo-BMT
| Recipient . | TT Proliferation (SI + SD),5-150 Time-Point of Analysis (wks) . | ||||
|---|---|---|---|---|---|
| t = 6 wks . | t = 10 wks . | t = 14 wks . | t = 32 wks . | t = 52 wks . | |
| UPN 272 | 0.6 ± 0.3 | 1.0 ± 0.3 | 1.0 ± 0.3 | 115-151 ± 3 | 26 ± 6 |
| UPN 279 | ND | 1.3 ± 0.4 | 2.4 ± 0.8 | 23 ± 7 | 36 ± 12 |
| UPN 298 | 1.1 ± 0.3 | 1.1 ± 0.3 | 1.5 ± 0.5 | ND | ND |
| UPN 301 | 1.1 ± 0.3 | ND | 1.1 ± 0.3 | 1.1 ± 0.3 | 2.1 ± 1 |
| UPN 275 | 0.8 ± 0.2 | 1.7 ± 0.6 | 5.7 ± 2.9 | 21 ± 6 | 16 ± 4 |
| UPN 285 | 1.1 ± 0.3 | 1.6 ± 0.5 | 2.0 ± 0.7 | 17 ± 6 | 27 ± 8 |
| Recipient . | TT Proliferation (SI + SD),5-150 Time-Point of Analysis (wks) . | ||||
|---|---|---|---|---|---|
| t = 6 wks . | t = 10 wks . | t = 14 wks . | t = 32 wks . | t = 52 wks . | |
| UPN 272 | 0.6 ± 0.3 | 1.0 ± 0.3 | 1.0 ± 0.3 | 115-151 ± 3 | 26 ± 6 |
| UPN 279 | ND | 1.3 ± 0.4 | 2.4 ± 0.8 | 23 ± 7 | 36 ± 12 |
| UPN 298 | 1.1 ± 0.3 | 1.1 ± 0.3 | 1.5 ± 0.5 | ND | ND |
| UPN 301 | 1.1 ± 0.3 | ND | 1.1 ± 0.3 | 1.1 ± 0.3 | 2.1 ± 1 |
| UPN 275 | 0.8 ± 0.2 | 1.7 ± 0.6 | 5.7 ± 2.9 | 21 ± 6 | 16 ± 4 |
| UPN 285 | 1.1 ± 0.3 | 1.6 ± 0.5 | 2.0 ± 0.7 | 17 ± 6 | 27 ± 8 |
Abbreviation: ND, not determined.
SI were calculated as SI = 3H incorporation of T cells + APC + antigen/3H incorporation of T cells and APC only; depicted is the mean SI of 3 experiments ± standard deviation (SD). The background values varied between 0.5 × 103 and 4.0 × 103 cpm and TT values varied between 0.7 × 103 and 84 × 103 cpm.
TT-stimulated T-cell lines were considered to be TT-specific when SI were 3 or greater (and cpm >5 × 103); these SI values are underlined.