Table 6. DNA Sequence Analysis of... | American Society of Hematology

Table 6.

DNA Sequence Analysis of TT-Specific T-Cell Clones Derived From HLA-Identical Sibling BMT Recipients (Group III) at 1 Year After BMT and From Their Respective Donors

No. of Clones	TCRBV		N-D-N	TCRBJ
D(UPN275)
1	8S1/2	$\underset{̲}{C}$ ASS	SGKH	EGF $\underset{̲}{FG}$	2S1
5	13S3	$\underset{̲}{C}$ ASSE	ALGQ	NYGYT $\underset{̲}{FG}$	1S2
1	13S3	$\underset{̲}{C}$ ASS	PLAGGL	IQY $\underset{̲}{FG}$	2S4
1	13S3	$\underset{̲}{C}$ ASSEA	LESGGFN	$\underset{̲}{FG}$	2S4
14^6-150	23S1	$\underset{̲}{C}$ ASSLG	PGRGA	YEQY $\underset{̲}{FG}$	2S7
UPN 275
1	2S1	$\underset{̲}{C}$ SAR	PGTALD	EQF $\underset{̲}{FG}$	2S1
4	5S2	$\underset{̲}{C}$ ASS	FKQ	TNEKLF $\underset{̲}{FG}$	1S4
2	5S4	$\underset{̲}{C}$ ASS	FLAGTG	TDTQY $\underset{̲}{FG}$	2S3
1	5S8	$\underset{̲}{C}$ ASS	HSSGGAP	GELF $\underset{̲}{FG}$	2S2
1	6S3	$\underset{̲}{C}$ ASS	SSGAT	YEQY $\underset{̲}{FG}$	2S7
4	7S2	$\underset{̲}{C}$ ASS	HLAGGP	ETQY $\underset{̲}{FG}$	2S5
1	13S1	$\underset{̲}{C}$ A	PIGGA	NYGYT $\underset{̲}{FG}$	1S2
1	13S3	$\underset{̲}{C}$ ASSE	DRGLES	YEQY $\underset{̲}{FG}$	2S7
1	15S1	$\underset{̲}{C}$ ATSD	SGTDMT	QPQH $\underset{̲}{FG}$	1S5
1	17S1	$\underset{̲}{C}$ ASS	IAAEGY	SYEQY $\underset{̲}{FG}$	2S7
1	21S3	$\underset{̲}{C}$ ASS	SMRAGG	TDTQY $\underset{̲}{FG}$	2S3
D(UPN285)
30	5S6	$\underset{̲}{C}$ ASS	GGLAGI^6-151	TDTQY $\underset{̲}{FG}$	2S3
1	6S7	$\underset{̲}{C}$ ASS	SGLAGGL	AKNIQY $\underset{̲}{FG}$	2S4
1	18S1	$\underset{̲}{C}$ ASS	LRA	DTQY $\underset{̲}{FG}$	2S3
UPN 285
2	1S1	$\underset{̲}{C}$ AS	GLG^6-151	STDTQY $\underset{̲}{FG}$	2S3
1	21S	$\underset{̲}{C}$ SAR	VPGTGGRGP	TDTQY $\underset{̲}{FG}$	2S3
2	5S1	$\underset{̲}{C}$ ASS	KEGAGA	T $\underset{̲}{FG}$	1S2
5	5S1	$\underset{̲}{C}$ ASS	YRGLAQG^6-151	TQY $\underset{̲}{FG}$	2S5
1	5S6	$\underset{̲}{C}$ ASS	PML	STDTQY $\underset{̲}{FG}$	2S3
1	6S5	$\underset{̲}{C}$ ASS	SGLAGGGR	TDTQY $\underset{̲}{FG}$	2S3
2	8S1/2	$\underset{̲}{C}$ ASS	VMGQGG	SPLH $\underset{̲}{FG}$	2S3
2	8S1/2	$\underset{̲}{C}$ ASS	FIGN	SPLH $\underset{̲}{FG}$	1S6
1	9S1	$\underset{̲}{C}$ ASSQ	PAA	GANVLT $\underset{̲}{FG}$	2S6
1	9S1	$\underset{̲}{C}$ ASS	PTGSG^6-151	TEAF $\underset{̲}{FG}$	1S1
1	12S3	$\underset{̲}{C}$ A	ITKGFTTT	TGELF $\underset{̲}{FG}$	2S2
2	14S1	$\underset{̲}{C}$ ASS	FCRST	YEQY $\underset{̲}{FG}$	2S7
11	18S1	$\underset{̲}{C}$ ASSP	SRTAI	GANVLT $\underset{̲}{FG}$	2S6

No. of Clones	TCRBV		N-D-N	TCRBJ
D(UPN275)
1	8S1/2	$\underset{̲}{C}$ ASS	SGKH	EGF $\underset{̲}{FG}$	2S1
5	13S3	$\underset{̲}{C}$ ASSE	ALGQ	NYGYT $\underset{̲}{FG}$	1S2
1	13S3	$\underset{̲}{C}$ ASS	PLAGGL	IQY $\underset{̲}{FG}$	2S4
1	13S3	$\underset{̲}{C}$ ASSEA	LESGGFN	$\underset{̲}{FG}$	2S4
14^6-150	23S1	$\underset{̲}{C}$ ASSLG	PGRGA	YEQY $\underset{̲}{FG}$	2S7
UPN 275
1	2S1	$\underset{̲}{C}$ SAR	PGTALD	EQF $\underset{̲}{FG}$	2S1
4	5S2	$\underset{̲}{C}$ ASS	FKQ	TNEKLF $\underset{̲}{FG}$	1S4
2	5S4	$\underset{̲}{C}$ ASS	FLAGTG	TDTQY $\underset{̲}{FG}$	2S3
1	5S8	$\underset{̲}{C}$ ASS	HSSGGAP	GELF $\underset{̲}{FG}$	2S2
1	6S3	$\underset{̲}{C}$ ASS	SSGAT	YEQY $\underset{̲}{FG}$	2S7
4	7S2	$\underset{̲}{C}$ ASS	HLAGGP	ETQY $\underset{̲}{FG}$	2S5
1	13S1	$\underset{̲}{C}$ A	PIGGA	NYGYT $\underset{̲}{FG}$	1S2
1	13S3	$\underset{̲}{C}$ ASSE	DRGLES	YEQY $\underset{̲}{FG}$	2S7
1	15S1	$\underset{̲}{C}$ ATSD	SGTDMT	QPQH $\underset{̲}{FG}$	1S5
1	17S1	$\underset{̲}{C}$ ASS	IAAEGY	SYEQY $\underset{̲}{FG}$	2S7
1	21S3	$\underset{̲}{C}$ ASS	SMRAGG	TDTQY $\underset{̲}{FG}$	2S3
D(UPN285)
30	5S6	$\underset{̲}{C}$ ASS	GGLAGI^6-151	TDTQY $\underset{̲}{FG}$	2S3
1	6S7	$\underset{̲}{C}$ ASS	SGLAGGL	AKNIQY $\underset{̲}{FG}$	2S4
1	18S1	$\underset{̲}{C}$ ASS	LRA	DTQY $\underset{̲}{FG}$	2S3
UPN 285
2	1S1	$\underset{̲}{C}$ AS	GLG^6-151	STDTQY $\underset{̲}{FG}$	2S3
1	21S	$\underset{̲}{C}$ SAR	VPGTGGRGP	TDTQY $\underset{̲}{FG}$	2S3
2	5S1	$\underset{̲}{C}$ ASS	KEGAGA	T $\underset{̲}{FG}$	1S2
5	5S1	$\underset{̲}{C}$ ASS	YRGLAQG^6-151	TQY $\underset{̲}{FG}$	2S5
1	5S6	$\underset{̲}{C}$ ASS	PML	STDTQY $\underset{̲}{FG}$	2S3
1	6S5	$\underset{̲}{C}$ ASS	SGLAGGGR	TDTQY $\underset{̲}{FG}$	2S3
2	8S1/2	$\underset{̲}{C}$ ASS	VMGQGG	SPLH $\underset{̲}{FG}$	2S3
2	8S1/2	$\underset{̲}{C}$ ASS	FIGN	SPLH $\underset{̲}{FG}$	1S6
1	9S1	$\underset{̲}{C}$ ASSQ	PAA	GANVLT $\underset{̲}{FG}$	2S6
1	9S1	$\underset{̲}{C}$ ASS	PTGSG^6-151	TEAF $\underset{̲}{FG}$	1S1
1	12S3	$\underset{̲}{C}$ A	ITKGFTTT	TGELF $\underset{̲}{FG}$	2S2
2	14S1	$\underset{̲}{C}$ ASS	FCRST	YEQY $\underset{̲}{FG}$	2S7
11	18S1	$\underset{̲}{C}$ ASSP	SRTAI	GANVLT $\underset{̲}{FG}$	2S6

Nomenclature according to Arden et al44 (TCRBV) and Toyanaga et al45 (TCRBJ). When the TCRBD could be determined, the amino acids encoded by the D segment are given in bold type. The underlined TCRBV/TCRBJ amino acids denote the boundaries of the CDR3 region.

F6-150

TCR sequences found more than 2 times; the number of sequences is depicted in italics.

F6-151

TCR CDR3 regions used as oligonucleotide probe for the longitudinal analysis of the TT-specific TCR repertoire.

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