Engraftment of Human Lin−CD34− Cells in NOD/SCID Mice After 4 Days of Culture
| . | Mouse No. . | |||||
|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | |
| Engrafted cells | 1 × 105 | 0.8 × 105 | 1.7 × 105 | 1.4 × 105 | 0.4 × 105 | 4.6 × 105 |
| CD34+ cells in engrafted cells | 1.5% | 0.4% | 10.9% | 8.7% | 11.2% | 2.2% |
| Human CD45+ in mouse BM | 15.2% | 0.4% | 65.4% | 2.3% | 0.5% | 0.8% |
| Human DNA in mouse BM | + | − | + | + | − | + |
| . | Mouse No. . | |||||
|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | |
| Engrafted cells | 1 × 105 | 0.8 × 105 | 1.7 × 105 | 1.4 × 105 | 0.4 × 105 | 4.6 × 105 |
| CD34+ cells in engrafted cells | 1.5% | 0.4% | 10.9% | 8.7% | 11.2% | 2.2% |
| Human CD45+ in mouse BM | 15.2% | 0.4% | 65.4% | 2.3% | 0.5% | 0.8% |
| Human DNA in mouse BM | + | − | + | + | − | + |
Human Lin−CD34− cells were cultured on HESS-5 stromal layers for 4 days and CD34 expression was measured by an FACS analysis. The total cell number and percentage of CD34+ cells transferred into NOD/SCID mice were indicated. Engraftment was determined by the detection of human CD45+cells using FACS and of HLA-DRB1 DNA by PCR 6 weeks after transplantation.