Table 2.

NOD/SCID engrafting ability of CD34+ cells after attachment and detachment

Matrix Presence of TS2/16 mAb Method of DetachmentEngrafted per Injected Mice % of Mice Engrafted*
 no  Pipetting  16/17  94  
FN no Pipetting  16/19  84  
FN yes  Pipetting 12/16  75  
RN no  Pipetting  5/6  83 
RN no  Peptides  9/11  82  
RN (16 h)  no Peptides  9/9  100 
Matrix Presence of TS2/16 mAb Method of DetachmentEngrafted per Injected Mice % of Mice Engrafted*
 no  Pipetting  16/17  94  
FN no Pipetting  16/19  84  
FN yes  Pipetting 12/16  75  
RN no  Pipetting  5/6  83 
RN no  Peptides  9/11  82  
RN (16 h)  no Peptides  9/9  100 
*

Values represent total mice engrafted/total mice injected × 100.

Cells were incubated in suspension.

Cells were incubated on matrix for 75 to 120 minutes.

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