Adult peripheral blood CD34+ cells express CXCR-4
| Source of Cells . | Overnight Incubation (Inc) . | CD34+/CXCR-4+ Cells* . | CD34+/CD38low/ CXCR-4+Cells* . | ||
|---|---|---|---|---|---|
| % of Positive Cells (Mean ± SD) . | Mean Fluorescence Intensity (AU) . | % of Positive Cells (Mean ± SD) . | Mean Fluorescence Intensity (AU) . | ||
| PB (n = 8) | − | 8.8 ± 3.6 | 50.2 ± 29.3 | 6.3 ± 2.9 | 42.3 ± 13.7 |
| + | 73.6 ± 12 | 460 ± 150 | 92.8 ± 4.7 | 605 ± 110 | |
| BM (n = 5) | − | 56 ± 12.3 | 91.2 ± 47.4 | 14.2 ± 8.3 | 48.2 ± 22.5 |
| + | 78.4 ± 17.7 | 338 ± 110 | 88.7 ± 5.3 | 208.2 ± 44.7 | |
| Source of Cells . | Overnight Incubation (Inc) . | CD34+/CXCR-4+ Cells* . | CD34+/CD38low/ CXCR-4+Cells* . | ||
|---|---|---|---|---|---|
| % of Positive Cells (Mean ± SD) . | Mean Fluorescence Intensity (AU) . | % of Positive Cells (Mean ± SD) . | Mean Fluorescence Intensity (AU) . | ||
| PB (n = 8) | − | 8.8 ± 3.6 | 50.2 ± 29.3 | 6.3 ± 2.9 | 42.3 ± 13.7 |
| + | 73.6 ± 12 | 460 ± 150 | 92.8 ± 4.7 | 605 ± 110 | |
| BM (n = 5) | − | 56 ± 12.3 | 91.2 ± 47.4 | 14.2 ± 8.3 | 48.2 ± 22.5 |
| + | 78.4 ± 17.7 | 338 ± 110 | 88.7 ± 5.3 | 208.2 ± 44.7 | |
AU indicates arbitrary units; PB, peripheral blood; BM, bone marrow.
CD34+ cells were purified from PB and BM, labeled, and analyzed for antigen expression with the use of a FACScalibur flow cytometer. Percentage of CD34+ and CD34+CD38low cells coexpressing CXCR-4 was calculated after gating on CD34+ or CD34+ CD38lowcells. Results are based on 5 to 8 independent experiments.