SDF-1 does not affect the colony formation by BM Inc− or Inc+ CD34+ cells
| SDF-1 . | BM Inc−CD34+ (No. of Colonies [mean ± SD])* . | BM Inc+ CD34+ (No. of Colonies [mean ± SD])* . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BFU-E . | CFU-G . | CFU-M . | CFU-GM . | CFU-Mix . | CFU Total . | BFU-E . | CFU-G . | CFU-M . | CFU-GM . | CFU-Mix . | CFU Total . | |
| 0 | 25 ± 4 | 44.5 ± 1.5 | 20 ± 3 | 23.5 ± 0.5 | 2.5 ± 0.5 | 115.5 ± 3.5 | 35 ± 3 | 66.5 ± 1.5 | 35.5 ± 3.5 | 20.5 ± 0.5 | 2.5 ± 1.5 | 160 ± 1 |
| .05 | 27 ± 2 | 47 ± 2 | 20.5 ± 6.5 | 21.5 ± 5.5 | 2 ± 1 | 104.5 ± 6.5 | 38 ± 5 | 70 ± 2 | 37.5 ± 6.5 | 22.5 ± 1.5 | 2.5 ± 0.5 | 170.5 ± 11 |
| .5 | 23.5 ± 3.5 | 46.5 ± 2.5 | 22.5 ± 7.5 | 17.5 ± 1.5 | 3 ± 1 | 113 ± 2 | 38.5 ± 3.5 | 68 ± 3 | 33 ± 3 | 22 ± 1 | 2.5 ± 1.5 | 164 ± 1 |
| 5 | 22 ± 1 | 45 ± 4 | 24.5 ± 0.5 | 19 ± 6 | 2.5 ± 1.5 | 113 ± 3 | 36 ± 1 | 69.5 ± 1.5 | 36 ± 4 | 23.5 ± 1.5 | 2.5 ± 0.5 | 167.5 ± 6 |
| 10 | 27.5 ± 1.5 | 44.5 ± 2.5 | 23.5 ± 2.5 | 20 ± 4 | 1.5 ± 1.5 | 117 ± 4 | 39.5 ± 1.5 | 67.5 ± 5.5 | 34 ± 1 | 26 ± 1 | 3 ± 1 | 170 ± 8 |
| SDF-1 . | BM Inc−CD34+ (No. of Colonies [mean ± SD])* . | BM Inc+ CD34+ (No. of Colonies [mean ± SD])* . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BFU-E . | CFU-G . | CFU-M . | CFU-GM . | CFU-Mix . | CFU Total . | BFU-E . | CFU-G . | CFU-M . | CFU-GM . | CFU-Mix . | CFU Total . | |
| 0 | 25 ± 4 | 44.5 ± 1.5 | 20 ± 3 | 23.5 ± 0.5 | 2.5 ± 0.5 | 115.5 ± 3.5 | 35 ± 3 | 66.5 ± 1.5 | 35.5 ± 3.5 | 20.5 ± 0.5 | 2.5 ± 1.5 | 160 ± 1 |
| .05 | 27 ± 2 | 47 ± 2 | 20.5 ± 6.5 | 21.5 ± 5.5 | 2 ± 1 | 104.5 ± 6.5 | 38 ± 5 | 70 ± 2 | 37.5 ± 6.5 | 22.5 ± 1.5 | 2.5 ± 0.5 | 170.5 ± 11 |
| .5 | 23.5 ± 3.5 | 46.5 ± 2.5 | 22.5 ± 7.5 | 17.5 ± 1.5 | 3 ± 1 | 113 ± 2 | 38.5 ± 3.5 | 68 ± 3 | 33 ± 3 | 22 ± 1 | 2.5 ± 1.5 | 164 ± 1 |
| 5 | 22 ± 1 | 45 ± 4 | 24.5 ± 0.5 | 19 ± 6 | 2.5 ± 1.5 | 113 ± 3 | 36 ± 1 | 69.5 ± 1.5 | 36 ± 4 | 23.5 ± 1.5 | 2.5 ± 0.5 | 167.5 ± 6 |
| 10 | 27.5 ± 1.5 | 44.5 ± 2.5 | 23.5 ± 2.5 | 20 ± 4 | 1.5 ± 1.5 | 117 ± 4 | 39.5 ± 1.5 | 67.5 ± 5.5 | 34 ± 1 | 26 ± 1 | 3 ± 1 | 170 ± 8 |
SDF-1 indicates stromal cell-derived factor-1; BM, bone marrow; BFU-E, erythroid; CFU-G, granulocytic; CFU-M, monocytic; CFU-GM, granulo-monocytic; CFU-Mix, multilineage. BM CD34+ cells were purified without (Inc−) or with (Inc+) overnight incubation on a plastic support and plated in duplicate at a density of 500 cells/mL on semisolid Stem α ID medium containing IL-3, IL-6, IL-11, SCF, G-CSF, GM-CSF, Epo, and various concentrations of SDF-1 (from 0 up to 10 ng/mL).
Colonies were scored on day 14, and results are based on 2 independent experiments.