Table 4. SDF-1 increases the percentage... | American Society of Hematology

Table 4.

SDF-1 increases the percentage of PB Inc⁺CD34⁺ cells in the S + G₂/M phases

Source of Cells	Incubation Time (h)	SDF-1 Treatment	% of Cells in Cell Cycle Phases^4-150 (Mean ± SD)
Source of Cells	Incubation Time (h)	SDF-1 Treatment	G₀/G₁	S + G₂/M
PB Inc⁻CD34⁺	0		98.6 ± 0.8	1.4 ± 0.8
	48	−	92.4 ± 0.6	7.6 ± 0.6
		+	90.9 ± 1.3	8.6 ± 0.8
	72	−	89.2 ± 2.6	9.4 ± 0.8
		+	88.5 ± 4.1	9.2 ± 0.4
PB Inc⁺ CD34⁺	0		98.3 ± 0.4	1.7 ± 0.4
	48	−	87.8 ± 0.6	12.1 ± 0.6
		+	66.8 ± 1.6	33.1 ± 1.6^4-151,^‡
	72	−	82.2 ± 1.2	16.8 ± 1.7
		+	69.7 ± 1.8	30.1 ± 2.2^4-151,^‡

Source of Cells	Incubation Time (h)	SDF-1 Treatment	% of Cells in Cell Cycle Phases^4-150 (Mean ± SD)
Source of Cells	Incubation Time (h)	SDF-1 Treatment	G₀/G₁	S + G₂/M
PB Inc⁻CD34⁺	0		98.6 ± 0.8	1.4 ± 0.8
	48	−	92.4 ± 0.6	7.6 ± 0.6
		+	90.9 ± 1.3	8.6 ± 0.8
	72	−	89.2 ± 2.6	9.4 ± 0.8
		+	88.5 ± 4.1	9.2 ± 0.4
PB Inc⁺ CD34⁺	0		98.3 ± 0.4	1.7 ± 0.4
	48	−	87.8 ± 0.6	12.1 ± 0.6
		+	66.8 ± 1.6	33.1 ± 1.6^4-151,^‡
	72	−	82.2 ± 1.2	16.8 ± 1.7
		+	69.7 ± 1.8	30.1 ± 2.2^4-151,^‡

PB CD34⁺ cells purified immediately after density gradient separation (Inc⁻) or after incubation on a plastic support (Inc⁺) were cultured (1 × 10⁵ cells/mL) in a serum- and cytokine-free medium with or without (control) SDF-1 (0.05 ng/mL). At various time points, cells were harvested by centrifugation, counted, stained with propidium iodide, and analyzed by flow cytometry.

F4-150

Based on three independent experiments.

F4-151

P = .004 versus SDF-1 untreated control cells.

‡

P < .0001 versus Inc⁻ cells.

View Large

This Feature Is Available To Subscribers Only