Table 2. LYST primers Name RT-PCR .... | American Society of Hematology

Table 2.

LYST primers

Name RT-PCR	Position 5′-3′ cDNA^*	Annealing Temperature (°C)	Purpose	PCR Products (bp)	Name Nested RT-PCR	Position 5′-3′ cDNA	Annealing Temperature (°C)	Purpose	PCR Products (bp)^‡
LYST1F LYST1R	127-146 1740-1755	51	Fragment 1	1628	LYST1′F^a LYST1′R	148-167 1651-1670	54	Fragment 1′	1522
LYST2F LYST2R	1215-1234 2789-2808	56	Fragment 2	1593	LYST2′F^a LYST2′R	1529-1547 2669-2688	52	Fragment 2′	1157
LYST3F LYST3R	2437-2456 4185-4201	52	Fragment 3	1764	LYST3′F^a LYST3′R	2641-2661 4057-4075	51	Fragment 3′	1434
LYST4F LYST4R	3311-3319 4855-4874	52	Fragment 4	1563	LYST4′F^a LYST4′R	3549-3566 4594-4613	50	Fragment 4′	1064
LYST5F LYST5R	4459-4476 6245-6265	50	Fragment 5	1806	LYST5′F^a LYST5′R	4523-4539 5957-5977	52	Fragment 5′	1454
LYST6F LYST6R	5800-5820 7619-7636	53	Fragment 6	1836	LYST6′F^a LYST6′R	5918-5938 7530-7550	52	Fragment 6′	1632
LYST7F LYST7R	7424-7444 9149-9169	52	Fragment 7	1745	LYST7′F^a LYST7′R	7477-7497 9038-9053	52	Fragment 7′	1576
LYST8F LYST8R	8950-8970 10314-10334	53	Fragment 8	1384	LYST8′F^a LYST8′R	9008-9027 10257-102761	53	Fragment 8′	1268
LYST9F LYST9R	10120-10137 11696-11716	54	Fragment 9	1596	LYST9′F^a LYST9′R	0184-10204 11677-11697	54	Fragment 9′	1513

Name RT-PCR	Position 5′-3′ cDNA^*	Annealing Temperature (°C)	Purpose	PCR Products (bp)	Name Nested RT-PCR	Position 5′-3′ cDNA	Annealing Temperature (°C)	Purpose	PCR Products (bp)^‡
LYST1F LYST1R	127-146 1740-1755	51	Fragment 1	1628	LYST1′F^a LYST1′R	148-167 1651-1670	54	Fragment 1′	1522
LYST2F LYST2R	1215-1234 2789-2808	56	Fragment 2	1593	LYST2′F^a LYST2′R	1529-1547 2669-2688	52	Fragment 2′	1157
LYST3F LYST3R	2437-2456 4185-4201	52	Fragment 3	1764	LYST3′F^a LYST3′R	2641-2661 4057-4075	51	Fragment 3′	1434
LYST4F LYST4R	3311-3319 4855-4874	52	Fragment 4	1563	LYST4′F^a LYST4′R	3549-3566 4594-4613	50	Fragment 4′	1064
LYST5F LYST5R	4459-4476 6245-6265	50	Fragment 5	1806	LYST5′F^a LYST5′R	4523-4539 5957-5977	52	Fragment 5′	1454
LYST6F LYST6R	5800-5820 7619-7636	53	Fragment 6	1836	LYST6′F^a LYST6′R	5918-5938 7530-7550	52	Fragment 6′	1632
LYST7F LYST7R	7424-7444 9149-9169	52	Fragment 7	1745	LYST7′F^a LYST7′R	7477-7497 9038-9053	52	Fragment 7′	1576
LYST8F LYST8R	8950-8970 10314-10334	53	Fragment 8	1384	LYST8′F^a LYST8′R	9008-9027 10257-102761	53	Fragment 8′	1268
LYST9F LYST9R	10120-10137 11696-11716	54	Fragment 9	1596	LYST9′F^a LYST9′R	0184-10204 11677-11697	54	Fragment 9′	1513

Position according to LYST sequence by Nagle et al13; accession number U67615.

†

T7-primer with promoter and sequence for initiation of translation $GCTAATACGACTCACTATAGGAACAGACCACC \underset{̲}{ATG} G .$ LYST primer sequence at 3′-position. The respective open reading frames start with the $\underset{̲}{ATG}$ codon.

‡

Length without T7 primer sequence, as described above.

RT-PCR, reverse transcription–polymerase chain reaction.

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