Table 1. Superoxide production in the... | American Society of Hematology

Table 1.

Superoxide production in the cell-free system

	Membranes From Patient With Control Cytosol	Cytosol From Patient With Control Membranes	Membranes and Cytosol From Control
JW (T341K)	0.04 (±0.03)	6.64 (±0.12)	6.67 (±0.47)
CE (C369R)	1.35 (±0.18)	7.65 (±0.34)	7.22 (±1.48)
MK (G408E)	0.05 (±0.02)	5.95 (±0.19)	4.83 (±0.19)
DG (E568K)	0.07 (±0.06)	5.86 (±0.25)	5.91 (±0.27)

	Membranes From Patient With Control Cytosol	Cytosol From Patient With Control Membranes	Membranes and Cytosol From Control
JW (T341K)	0.04 (±0.03)	6.64 (±0.12)	6.67 (±0.47)
CE (C369R)	1.35 (±0.18)	7.65 (±0.34)	7.22 (±1.48)
MK (G408E)	0.05 (±0.02)	5.95 (±0.19)	4.83 (±0.19)
DG (E568K)	0.07 (±0.06)	5.86 (±0.25)	5.91 (±0.27)

Values are given in μmol $O_{2}^{-}$ /min (mean ± SD; n = 3). Neutrophil membranes (10 μg) and cytosol (200 μg) were incubated at 28°C in oxidase buffer, as previously described, in the presence of 60 μmol/L cytochrome c. After 2 minutes of incubation, SDS (100 μmol/L) and GTPγS (10 μmol/L) were added. After another 5 minutes, NADPH (250 μmol/L) was added, and the rate of cytochrome c reduction was determined by the change in absorbance at 550 nm.

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