Oxygen consumption of membranes without cytosol
| Membranes . | Oxygen Consumption (nmol/O2/min mg) membrane protein . |
|---|---|
| Con (n = 3) | 125 ± 7.5 |
| JW (T341K) | 0 |
| CE (C369R) | 3.3 |
| MK (G408E) | 0 |
| DG (E568K) | 0 |
| Membranes . | Oxygen Consumption (nmol/O2/min mg) membrane protein . |
|---|---|
| Con (n = 3) | 125 ± 7.5 |
| JW (T341K) | 0 |
| CE (C369R) | 3.3 |
| MK (G408E) | 0 |
| DG (E568K) | 0 |
Control is mean ± SD. Patients' or control neutrophil membranes (6 μg) were solubilized with octylglucoside and incubated with phosphatidyl choline/phosphatidic acid for 30 minutes. Oxygen consumption was measured in 500 μL after addition of 250 μmol/L NADPH.