Table 1. Yield of total, divided, and... | American Society of Hematology

Table 1.

Yield of total, divided, and undivided cells from all in vitro cell tracking experiments

Exp.No.	Recipient Genotype	Donor Genotype	Phenotype of Input CFSE⁺ Cells	No. of Input CFSE⁺ Cells (×10⁴)	Growth Factors Used	Duration of Culture in Days	Yield of Cultured Cells
Exp.No.	Recipient Genotype	Donor Genotype	Phenotype of Input CFSE⁺ Cells	No. of Input CFSE⁺ Cells (×10⁴)	Growth Factors Used	Duration of Culture in Days	Total Cells (×10⁴)	Expansion	Undivided Cells ×10⁴ (% of total)	Divided Cells ×10⁴ (% of total)
1	W⁴¹	Pep3b	Lin⁻	1.6	FL, SF, IL-11	3	12	0.8	0.1 (7)	1 (93)
6	B6C3	PepC3	Lin⁻	30	FL, SF, IL-11	3	32	1.1	1 (3)	31 (97)
							mean	1.0	(5)	(95)
2	B6	Pep3b	LD	18	FL, SF, IL-11	4	69	3.8	3 (4)	66 (96)
5	B6C3	PepC3	LD	50	FL, SF, H-IL-6	4	240	4.8	11 (5)	230 (95)
7	B6C3	PepC3	LD	50	FL, SF, H-IL-6	4	63	1.3	7 (11)	56 (89)
							mean ± SEM	3.3 ± 1.0	(7 ± 2)	(93 ± 2)
8	B6C3	PepC3	Lin⁻	20	FL, SF, IL-11	4	150	7.5	2 (1)	140 (99)
3	W⁴¹	Pep3b	Lin⁻Sca-1⁺	10	FL, SF, H-IL-6	10	630	63	0.025 (0.004)	630 (99.9)
4	Gpi-1^a	Gpi-1^b	Lin⁻Sca-1⁺	8	FL, SF, H-IL-6	10	510	64	0.035 (0.007)	510 (99.9)
							mean	63	(0.005)	(99.9)

Exp.No.	Recipient Genotype	Donor Genotype	Phenotype of Input CFSE⁺ Cells	No. of Input CFSE⁺ Cells (×10⁴)	Growth Factors Used	Duration of Culture in Days	Yield of Cultured Cells
Exp.No.	Recipient Genotype	Donor Genotype	Phenotype of Input CFSE⁺ Cells	No. of Input CFSE⁺ Cells (×10⁴)	Growth Factors Used	Duration of Culture in Days	Total Cells (×10⁴)	Expansion	Undivided Cells ×10⁴ (% of total)	Divided Cells ×10⁴ (% of total)
1	W⁴¹	Pep3b	Lin⁻	1.6	FL, SF, IL-11	3	12	0.8	0.1 (7)	1 (93)
6	B6C3	PepC3	Lin⁻	30	FL, SF, IL-11	3	32	1.1	1 (3)	31 (97)
							mean	1.0	(5)	(95)
2	B6	Pep3b	LD	18	FL, SF, IL-11	4	69	3.8	3 (4)	66 (96)
5	B6C3	PepC3	LD	50	FL, SF, H-IL-6	4	240	4.8	11 (5)	230 (95)
7	B6C3	PepC3	LD	50	FL, SF, H-IL-6	4	63	1.3	7 (11)	56 (89)
							mean ± SEM	3.3 ± 1.0	(7 ± 2)	(93 ± 2)
8	B6C3	PepC3	Lin⁻	20	FL, SF, IL-11	4	150	7.5	2 (1)	140 (99)
3	W⁴¹	Pep3b	Lin⁻Sca-1⁺	10	FL, SF, H-IL-6	10	630	63	0.025 (0.004)	630 (99.9)
4	Gpi-1^a	Gpi-1^b	Lin⁻Sca-1⁺	8	FL, SF, H-IL-6	10	510	64	0.035 (0.007)	510 (99.9)
							mean	63	(0.005)	(99.9)

The duration of culture shown is the total period of incubation of the cells including the extra day involved in the CFSE labeling procedure prior to isolating the “input” CFSE⁺ cells. Some CFSE⁺ cells in experiments 5, 7, and 8 were injected directly after labeling (day 1) into irradiated mice. In experiment No. 8, CRU assays were not performed on the cultured cells.

At the end of the culture period, but before assessing undivided and divided cells, cell counts were performed. The number of cells in each fraction was then calculated by multiplying the relative proportion of cells in each fraction (% of total) by the total number of cells recovered, assuming 83% recovery due to the gap used to separate divided and undivided cells (see “Materials and Methods”). Mean values for experiments with similar types of input cells cultured for similar periods are shown (ie, for experiments 1 and 6; 2, 5, and 7; and 3 and 4).

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