Effects of SDF-1 on the growth of CFU-MK-derived colonies
| Experiment no. . | Growth Factor . | No. MK Colonies (per 105 cells) . |
|---|---|---|
| 1 | TPO | 21 ± 1 |
| TPO + SDF-1 | 31 ± 1* | |
| 2 | TPO | 19 ± 1 |
| TPO + SDF-1 | 34 ± 5† | |
| 3 | TPO | 25 ± 5 |
| SDF-1 | 2 ± 1 | |
| TPO + SDF-1 | 34 ± 1‡ | |
| 4 | TPO | 17 ± 2 |
| TPO + SDF-1 | 38 ± 2* | |
| Summary | TPO | 21 ± 1.3 |
| TPO + SDF-1 | 34 ± 1.01-153 |
| Experiment no. . | Growth Factor . | No. MK Colonies (per 105 cells) . |
|---|---|---|
| 1 | TPO | 21 ± 1 |
| TPO + SDF-1 | 31 ± 1* | |
| 2 | TPO | 19 ± 1 |
| TPO + SDF-1 | 34 ± 5† | |
| 3 | TPO | 25 ± 5 |
| SDF-1 | 2 ± 1 | |
| TPO + SDF-1 | 34 ± 1‡ | |
| 4 | TPO | 17 ± 2 |
| TPO + SDF-1 | 38 ± 2* | |
| Summary | TPO | 21 ± 1.3 |
| TPO + SDF-1 | 34 ± 1.01-153 |
Murine marrow cells were incubated in 0.275% agar in IMDM supplemented with 15% horse serum, 5 × 10−5 mol/L β-mercaptoethanol, and cytokines with or without 200 ng/mL SDF-1. The number of colonies was enumerated on day 5 using an inverted microscope. The combination of TPO, IL-3, IL-6, and SCF provided a measure of the total number of CFU-MK in the cells plated. The data represent the mean ± SEM of 4 experiments of triplicate plates. Summation of all 12 plates for each condition is shown on the lower two lines.
P < 0.01.
P < 0.02.
P < 0.05.
P < 0.001 (Student's 2-tailed t-test for paired values) compared to cultures containing TPO alone.