Table 5. The effect of SDF-1 on the... | American Society of Hematology

Table 5.

The effect of SDF-1 on the growth of CFU-MK colonies generated from cells at each purification step

Experiment No.		Whole Marrow	Density Gradient	Lineage Depletion	FACS Sort
1	TPO	19 ± 3	32 ± 4	310 ± 72	5700 ± 129
	TPO + SDF-1	34 ± 5^5-151	96 ± 16^5-152	420 ± 100^5-152	7900 ± 461^5-152
2	TPO	24 ± 0.3	43 ± 6	370 ± 11	4000 ± 400
	TPO + SDF-1	30 ± 0.5^5-151	75 ± 9^5-150	500 ± 40^5-150	7300 ± 175^5-151
Summary	TPO	22 ± 1.3	39 ± 3.3	340 ± 22	4900 ± 540
	TPO + SDF-1	32 ± 1.4^5-150	86 ± 6.7^5-153	460 ± 34^5-152	7600 ± 210^5-153

Experiment No.		Whole Marrow	Density Gradient	Lineage Depletion	FACS Sort
1	TPO	19 ± 3	32 ± 4	310 ± 72	5700 ± 129
	TPO + SDF-1	34 ± 5^5-151	96 ± 16^5-152	420 ± 100^5-152	7900 ± 461^5-152
2	TPO	24 ± 0.3	43 ± 6	370 ± 11	4000 ± 400
	TPO + SDF-1	30 ± 0.5^5-151	75 ± 9^5-150	500 ± 40^5-150	7300 ± 175^5-151
Summary	TPO	22 ± 1.3	39 ± 3.3	340 ± 22	4900 ± 540
	TPO + SDF-1	32 ± 1.4^5-150	86 ± 6.7^5-153	460 ± 34^5-152	7600 ± 210^5-153

Cells separated in each purification step were cultured as described in Table 1 in the presence of TPO (25 ng/mL) with or without SDF-1 (200 ng/mL). CFU-MK-derived colonies were counted on day 5.

P < 0.01.

P < 0.02.

P < 0.05.

P < 0.005 (Student's 2-tailed t-test for paired values) compared to cultures containing TPO alone. The data represent the mean (±SEM) of triplicate plates expressed per 10⁵ cells plated. Summation of the data from both experiments is shown in the last two lines.

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