Table 6. The inhibition of the effect of... | American Society of Hematology

Table 6.

The inhibition of the effect of SDF-1 by T22 in serum free cultures

	No. MK Colonies (per 10⁵ cells)
	TPO	TPO + SDF-1	TPO + T22
whole marrow cells	18 ± 3	34 ± 7^6-150	21 ± 3^NS
Density gradient	42 ± 6	74 ± 12^6-150	50 ± 2^NS
FACS sorting
exp. 1	500 ± 25	800 ± 100^6-150	525 ± 100^NS
exp. 2	400 ± 100	900 ± 75^6-150	425 ± 100^NS

	No. MK Colonies (per 10⁵ cells)
	TPO	TPO + SDF-1	TPO + T22
whole marrow cells	18 ± 3	34 ± 7^6-150	21 ± 3^NS
Density gradient	42 ± 6	74 ± 12^6-150	50 ± 2^NS
FACS sorting
exp. 1	500 ± 25	800 ± 100^6-150	525 ± 100^NS
exp. 2	400 ± 100	900 ± 75^6-150	425 ± 100^NS

Cells in each purification step were plated in serum-depleted medium with TPO and SDF-1 with or without T22. The results represent the number of CFU-MK-derived colonies (mean ± SEM of triplicate plates).

F6-150

P < 0.05 compared to TPO alone; NS not significant compared to cultures containing TPO alone.

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