The effect of 7E3 F(ab′)2 and LM609 on hemodynamic behavior of SS RBC in PAF-treated mesocecum vasculature
| A: 7E3 . | |||||
|---|---|---|---|---|---|
| Experiment . | No. . | PRU (mm Hg/mL/min/g) . | ΔPRU (%) . | Tpf (s) . | |
| Ringer . | RBC . | ||||
| 1. Control Ab/PAF + Control Ab/SS RBC | 5 | 7.3 ± 1.6 | 10.1 ± 2.8 | 37.8 ± 12.7 | 97.0 ± 9.7 |
| 2. 7E3/PAF + 7E3/SS RBC | 5 | 6.2 ± 1.6 | 7.7 ± 2.3 | 23.5 ± 7.2* | 62.0 ± 5.7† |
| 3. 10E5/PAF + 10E5/SS RBC | 4 | 8.8 ± 4.3 | 12.1 ± 6.7 | 34.7 ± 6.8 | 105.0 ± 31.0 |
| A: 7E3 . | |||||
|---|---|---|---|---|---|
| Experiment . | No. . | PRU (mm Hg/mL/min/g) . | ΔPRU (%) . | Tpf (s) . | |
| Ringer . | RBC . | ||||
| 1. Control Ab/PAF + Control Ab/SS RBC | 5 | 7.3 ± 1.6 | 10.1 ± 2.8 | 37.8 ± 12.7 | 97.0 ± 9.7 |
| 2. 7E3/PAF + 7E3/SS RBC | 5 | 6.2 ± 1.6 | 7.7 ± 2.3 | 23.5 ± 7.2* | 62.0 ± 5.7† |
| 3. 10E5/PAF + 10E5/SS RBC | 4 | 8.8 ± 4.3 | 12.1 ± 6.7 | 34.7 ± 6.8 | 105.0 ± 31.0 |
| B: LM609 . | |||||
|---|---|---|---|---|---|
| Experiment . | No. . | PRU (mm Hg/mL/min/g) . | ΔPRU (%) . | Tpf (s) . | |
| Ringer . | RBC . | ||||
| 1. Control Ab/PAF/SS RBC | 4 | 9.3 ± 2.4 | 14.0 ± 3.5 | 50.3 ± 8.7 | 117.5 ± 9.6 |
| 2. LM609/PAF/SS RBC | 4 | 7.6 ± 2.4 | 9.6 ± 3.5 | 25.1 ± 8.0‡ | 82.0 ± 9.32-153 |
| B: LM609 . | |||||
|---|---|---|---|---|---|
| Experiment . | No. . | PRU (mm Hg/mL/min/g) . | ΔPRU (%) . | Tpf (s) . | |
| Ringer . | RBC . | ||||
| 1. Control Ab/PAF/SS RBC | 4 | 9.3 ± 2.4 | 14.0 ± 3.5 | 50.3 ± 8.7 | 117.5 ± 9.6 |
| 2. LM609/PAF/SS RBC | 4 | 7.6 ± 2.4 | 9.6 ± 3.5 | 25.1 ± 8.0‡ | 82.0 ± 9.32-153 |
Values are mean ± SD. In these experiments, the vasculature was first incubated with either control (OC125) or 7E3 F(ab′)2antibodies (50 μg/mL, 5 mL) for 30 minutes followed by infusion of PAF (200 pg/mL, 40 mL) containing a given antibody. In one control experiment, the preparation was preinfused with PAF only.
Abbreviations: ΔPRU, PRU RBC/PRU Ringer; Tpf, pressure-flow recovery time to the baseline level.
P < .013 and P < .05 compared with ΔPRU for control Ab and 10E5 groups, respectively.
P < .0003 and P < .02 compared with Tpf for control Ab and 10E5 groups, respectively.
P < .006 compared with respective values for the control antibody group.
P < .02 compared with respective values for the control antibody group.