Comparison of ATP and its stable analogues ATPγS and met-ATP on actin response and chemiluminescence
Stimulus
.
f-actin
.
Chemiluminescence
.
Control
1.00 (± 0.00)
5.16 (± 0.64)
ATP
1.57 (± 0.11)
395.67 (± 41.53)
ATPγS
(± 0.13)
(± 59.27)
met-ATP
(± 0.11)
(± 46.30)
Stimulus
.
f-actin
.
Chemiluminescence
.
Control
1.00 (± 0.00)
5.16 (± 0.64)
ATP
1.57 (± 0.11)
395.67 (± 41.53)
ATPγS
(± 0.13)
(± 59.27)
met-ATP
(± 0.11)
(± 46.30)
Eosinophils were stimulated without or with 100 μmol/mL ATP, ATPγS, or met-ATP. The relative f-actin content was taken after 25 seconds. Chemiluminescence response is given as integral (counts × 106) after 60 minutes. Data are means ± SEM (n = 5).