TPA treatment of CD34+ cells before EPO- and SCF- addition inhibits the cytokine-induced erythroid differentiation
| Treatment . | % Positive Cells . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0-24 Hours . | 24-144 Hours . | CD34 . | GPA . | CD71 . | CD13 . | CD14 . | HLA-DR . | CD83 . | CD86 . | CD41 . |
| Medium | EPO + SCF | 9 ± 4 | 40 ± 5 | 82 ± 3 | 16 ± 4 | 3 ± 1 | 18 ± 4 | 1 ± 1 | 3 ± 3 | 7 ± 3 |
| TPA 100 nmol/L | EPO + SCF | 25 ± 5 | 2 ± 1 | 32 ± 7 | 55 ± 8 | 6 ± 2 | 67 ± 5 | 14 ± 4 | 37 ± 11 | 20 ± 3 |
| TPA 10 nmol/L | EPO + SCF | 17 ± 5 | 2 ± 1 | 19 ± 7 | 56 ± 4 | 4 ± 1 | 62 ± 11 | 6 ± 3 | 23 ± 8 | 21 ± 0 |
| Treatment . | % Positive Cells . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0-24 Hours . | 24-144 Hours . | CD34 . | GPA . | CD71 . | CD13 . | CD14 . | HLA-DR . | CD83 . | CD86 . | CD41 . |
| Medium | EPO + SCF | 9 ± 4 | 40 ± 5 | 82 ± 3 | 16 ± 4 | 3 ± 1 | 18 ± 4 | 1 ± 1 | 3 ± 3 | 7 ± 3 |
| TPA 100 nmol/L | EPO + SCF | 25 ± 5 | 2 ± 1 | 32 ± 7 | 55 ± 8 | 6 ± 2 | 67 ± 5 | 14 ± 4 | 37 ± 11 | 20 ± 3 |
| TPA 10 nmol/L | EPO + SCF | 17 ± 5 | 2 ± 1 | 19 ± 7 | 56 ± 4 | 4 ± 1 | 62 ± 11 | 6 ± 3 | 23 ± 8 | 21 ± 0 |
CD34+ cells were cultured in medium alone or in the presence of 100 or 10 nmol/L TPA for 24 hours. EPO (5 U/mL) and SCF (50 ng/mL) were added, and this was followed by 6 days of incubation before staining with the indicated mAbs. Mean ± SEM of 3 to 7 experiments. GPA, glycophorin A.