Effect of fMLP and PMA on EA rosetting by CD32A expressing CHO cell transfectants
| Treatment . | % Rosetting (mean ± SD) . |
|---|---|
| 4°C | 55.3 ± 4.04 |
| 4°C + IV.3 | 1.33 ± 1.26 |
| 37°C | 42.2 ± 9.38 |
| 37°C + IV.3 | 1.17 ± 1.26 |
| fMLP | 46.8 ± 4.54 |
| fMLP + IV.3 | 2.5 ± 1.5 |
| PMA | 52.0 ± 11.3 |
| PMA + IV.3 | 1.33 ± 1.53 |
| Treatment . | % Rosetting (mean ± SD) . |
|---|---|
| 4°C | 55.3 ± 4.04 |
| 4°C + IV.3 | 1.33 ± 1.26 |
| 37°C | 42.2 ± 9.38 |
| 37°C + IV.3 | 1.17 ± 1.26 |
| fMLP | 46.8 ± 4.54 |
| fMLP + IV.3 | 2.5 ± 1.5 |
| PMA | 52.0 ± 11.3 |
| PMA + IV.3 | 1.33 ± 1.53 |
CHO cell transfectants expressing CD32A were incubated with or without fMLP (10−6 M) or PMA (50 ng/mL) for 1 h at 37°C and allowed to form rosettes with EA in ice for 2 h. Fab fragments of IV.3 (10 μg/mL) was included in some experiments. Results are expressed as mean of three experiments. Untransfected CHO cells did not form EA rosettes.
CHO, Chinese hamster ovary cells; fMLP, formyl-methionyl-leucyl-phenylalanine.