Absence of proliferation after exposure of B-CLL cells to Gaμ-ab or Gaδ-ab
| Stimulus† . | Cell Cycle Analysis (phase %)* . | 3H Thymidine Uptake (cpm) . | ||||
|---|---|---|---|---|---|---|
| G0/G1 . | S . | G2/M . | 24 h . | 48 h . | 72 h . | |
| NGI | 94 | 5 | 1 | 200 | 350 | 220 |
| Gaμ-ab | 93 | 4 | 3 | 80 | 60 | 20 |
| Gaδ-ab | 90 | 8 | 2 | 420 | 500 | 520 |
| PMA + A23187 | 63 | 28 | 9 | 12 000 | 16 000 | 8000 |
| Stimulus† . | Cell Cycle Analysis (phase %)* . | 3H Thymidine Uptake (cpm) . | ||||
|---|---|---|---|---|---|---|
| G0/G1 . | S . | G2/M . | 24 h . | 48 h . | 72 h . | |
| NGI | 94 | 5 | 1 | 200 | 350 | 220 |
| Gaμ-ab | 93 | 4 | 3 | 80 | 60 | 20 |
| Gaδ-ab | 90 | 8 | 2 | 420 | 500 | 520 |
| PMA + A23187 | 63 | 28 | 9 | 12 000 | 16 000 | 8000 |
Results are from 1 representative experiment of 3 performed on patients with B-CLL (patients A, E, L). 3H thymidine uptake values are means of triplicate cultures.
Cell-cycle analysis of the cells was performed after 48 h of culture, as described in “Materials and Methods.”
B-CLL cells were incubated with Gaμ-ab (10 μg/mL), Gaδ-ab (10 μg/mL), PMA (10 ng/mL) plus A23187 (5 ng/mL). Variations in the concentration of both the reagents (from 50 ng/mL to 80 μg/mL) did not result in any significant change in the result observed.