Recruitment into cell cycle induced by exposure of peripheral blood B cells to Gaμ-ab and Gaδ-ab
| Stimulus† . | Cell Cycle Analysis (phase %)* . | Apoptosis . | ||
|---|---|---|---|---|
| G0/G1 . | S . | G2/M . | % Annexin V-FITC Cells . | |
| NGI | 98 | 2 | 0 | 10 |
| Gaμ-ab | 82 | 17 | 1 | 5 |
| Gaδ-ab | 83 | 16 | 1 | 5 |
| SAC | 78 | 20 | 2 | 8 |
| Stimulus† . | Cell Cycle Analysis (phase %)* . | Apoptosis . | ||
|---|---|---|---|---|
| G0/G1 . | S . | G2/M . | % Annexin V-FITC Cells . | |
| NGI | 98 | 2 | 0 | 10 |
| Gaμ-ab | 82 | 17 | 1 | 5 |
| Gaδ-ab | 83 | 16 | 1 | 5 |
| SAC | 78 | 20 | 2 | 8 |
Results are from 1 representative experiment of 3 performed on different peripheral blood B cells. Apoptotic cells were detected by Annexin-V FITC staining.
Cell-cycle analysis of the cells was performed after 48 h of culture, as described in “Materials and Methods.”
Peripheral blood B cells were incubated with Gaμ-ab (10 μg/mL), Gaδ-ab (10 μg/mL), SAC (1:10000 vol/vol). Variations in the concentration of both the reagents (from 50 ng/mL to 80 μg/mL) did not result in any significant change in the result observed.