Quantified expression of CD100, CD148, and CD45 in CD38+CD138− SBL and LBL populations of activated peripheral blood B lymphocytes
. | LBL . | SBL . |
---|---|---|
CD100 | 11 600 | 3 600 |
(4 700-19 600) | (2 200-5 500) | |
CD148 | 6 800* | 1 700* |
(1 700-12 000) | (500-2 900) | |
CD45 | 115 000 | 85 400 |
(65 000-170 000) | (53 000-120 000) |
. | LBL . | SBL . |
---|---|---|
CD100 | 11 600 | 3 600 |
(4 700-19 600) | (2 200-5 500) | |
CD148 | 6 800* | 1 700* |
(1 700-12 000) | (500-2 900) | |
CD45 | 115 000 | 85 400 |
(65 000-170 000) | (53 000-120 000) |
LBL indicates large B lymphocytes; SBL, small B lymphocytes. LBL and SBL populations were defined according to Figure 1. The number of antigenic sites per cell was determined by means of the QIFIKIT procedure and flow cytometry analysis described in “Materials and Methods,” with BD16, 143-41, and O509 mAbs specific for CD100, CD148, and CD45, respectively. Results are expressed as the mean (range) from at least five experiments performed with different donors.
Only one subpopulation expressed CD148.