Quantified expression of CD100, CD45, and CD148 and their associated PTP activities in B-cell lines at various stages of differentiation
| Cell Line . | CD100 . | CD45 . | CD148 . | |||
|---|---|---|---|---|---|---|
| Sites/Cell . | PTP . | Sites/Cell . | PTP . | Sites/Cell . | PTP . | |
| Pre-B | ||||||
| Nalm-6 | 2 000 | nt | 1 500 | nt | nt | nt |
| Reh-6 | 2 300 | 0.09 | 31 000 | 0.51 | nt | nt |
| Lymphoblastoid | ||||||
| Daudi | 7 100 | nt | 160 200 | nt | 2 300 | nt |
| Raji | 1 000 | 0.15 | 24 900 | 1.50 | 0 | nt |
| Ramos | 3 800 | 0.15 | 65 000 | 1.03 | nt | nt |
| EBV-activated | ||||||
| DZ | 21 800 | 0.13 | 41 900 | 0.68 | nt | nt |
| JY | 10 800 | 0.05 | 20 800 | 0.40 | nt | nt |
| Sanchez | 12 500 | NS | 42 500 | 0.30 | 1 000 | 0.20 |
| Pre-plasma | ||||||
| Eskol | 15 000 | 0.12 | 87 000 | 1.90 | 5 200 | 0.38 |
| Plasma | ||||||
| LP-1 | 19 800 | 0.12 | 0 | NS | 0 | nt |
| RPMI-8226 | 4 100 | NS | 3 700 | 0.05 | 0 | NS |
| U-266 | 7 300 | 0.54 | 200 | NS | 4 800 | 0.63 |
| XG-1 | 8 900 | 0.26 | 85 500 | 1.10 | 12 500 | 1.24 |
| XG-2 | 34 300 | NS | 300 | nt | 0 | nt |
| Cell Line . | CD100 . | CD45 . | CD148 . | |||
|---|---|---|---|---|---|---|
| Sites/Cell . | PTP . | Sites/Cell . | PTP . | Sites/Cell . | PTP . | |
| Pre-B | ||||||
| Nalm-6 | 2 000 | nt | 1 500 | nt | nt | nt |
| Reh-6 | 2 300 | 0.09 | 31 000 | 0.51 | nt | nt |
| Lymphoblastoid | ||||||
| Daudi | 7 100 | nt | 160 200 | nt | 2 300 | nt |
| Raji | 1 000 | 0.15 | 24 900 | 1.50 | 0 | nt |
| Ramos | 3 800 | 0.15 | 65 000 | 1.03 | nt | nt |
| EBV-activated | ||||||
| DZ | 21 800 | 0.13 | 41 900 | 0.68 | nt | nt |
| JY | 10 800 | 0.05 | 20 800 | 0.40 | nt | nt |
| Sanchez | 12 500 | NS | 42 500 | 0.30 | 1 000 | 0.20 |
| Pre-plasma | ||||||
| Eskol | 15 000 | 0.12 | 87 000 | 1.90 | 5 200 | 0.38 |
| Plasma | ||||||
| LP-1 | 19 800 | 0.12 | 0 | NS | 0 | nt |
| RPMI-8226 | 4 100 | NS | 3 700 | 0.05 | 0 | NS |
| U-266 | 7 300 | 0.54 | 200 | NS | 4 800 | 0.63 |
| XG-1 | 8 900 | 0.26 | 85 500 | 1.10 | 12 500 | 1.24 |
| XG-2 | 34 300 | NS | 300 | nt | 0 | nt |
PTP indicates protein tyrosine phosphatase. The numbers of antigenic sites per cell were quantified by means of the QIFIKIT procedure and flow cytometry analysis with BD16, O509, and 143-41 mAbs, respectively. All cell lines were negative for CD101 expression, as evaluated with the BB27 mAb. PTP activity was assayed on cell lysates after immunoprecipitation with the specific mAbs BD16, BJ45 (anti-CD45), a mixture (1:1) of 143-41 and A3 (anti-CD148), and BB27. All experimental procedures are described in “Materials and Methods.” PTP activity is expressed as the absorbance at 405 nm. CD101 negative control values ranged from 0.00 to 0.04 (mean = 0.02).
NS, not significant (<.05); nt, not tested.