Effect of CD45 immunodepletion on PTP activity immunoprecipitated with CD100 in CD38+CD138− cells sorted from activated peripheral blood and tonsillar B lymphocytes
| . | Immunoprecipitates . | ||
|---|---|---|---|
| CD101 . | CD100 . | CD45 . | |
| Peripheral blood cells | |||
| No depletion | NS | 0.07 | 1.14 |
| Control depletion (CD101) | ND | 0.06 | 0.67 |
| CD45 depletion | ND | NS | 0.13 |
| Tonsillar cells | |||
| No depletion | NS | 0.08 | 0.52 |
| Control depletion (CD101) | ND | 0.07 | 0.36 |
| CD45 depletion | ND | NS | 0.08 |
| . | Immunoprecipitates . | ||
|---|---|---|---|
| CD101 . | CD100 . | CD45 . | |
| Peripheral blood cells | |||
| No depletion | NS | 0.07 | 1.14 |
| Control depletion (CD101) | ND | 0.06 | 0.67 |
| CD45 depletion | ND | NS | 0.13 |
| Tonsillar cells | |||
| No depletion | NS | 0.08 | 0.52 |
| Control depletion (CD101) | ND | 0.07 | 0.36 |
| CD45 depletion | ND | NS | 0.08 |
Nongranular CD138− cells were isolated with the use of cell sorting from populations induced by activation of peripheral blood (donor 8) or tonsillar B lymphocytes, as defined in Figure 1. Sorted cells were CD100+CD38+CD138−. PTP activity was assayed on CD101 (negative control value), CD100, and CD45 immunoprecipitates obtained from depleted or undepleted cell lysates (2 × 106 peripheral blood cells and 1.5 × 106 tonsillar cells per 100 μL of lysates). All procedures are described in “Materials and Methods.” Results are expressed as in Tables 3 and 4.
NS, not significant; ND, not determined.