Table 2.

As2O3-induced cell-cycle effects and apoptosis

% Cells
sub-G1G1S G2/M
HL-60/neo 
 Control  1.4 ± 1.2  33.0 ± 3.3  16.8 ± 2.5 35.5 ± 3.6  
 1.0 μmol/L As2O3 17.0 ± 2.6  40.3 ± 4.5  12.6 ± 3.5 30.3 ± 5.6  
 2.0 μmol/L As2O3 24.6 ± 3.5  39.3 ± 2.1  12.5 ± 2.7 27.1 ± 6.3  
HL-60/Bcr-abl  
 Control 1.5 ± 1.8  40.8 ± 5.6  18.1 ± 2.1 30.7 ± 2.5  
 1.0 μmol/L As2O3 12.0 ± 3.6  32.2 ± 4.1  8.1 ± 3.4 51.4 ± 4.9* 
 2.0 μmol/L As2O3 21.1 ± 5.5  20.7 ± 3.6  9.4 ± 4.7 53.2 ± 5.6* 
K562  
 Control  3.1 ± 1.9 38.2 ± 2.9  16.7 ± 1.9  36.7 ± 3.1  
 1.0 μmol/L As2O3 10.5 ± 2.5 26.1 ± 5.4  12.4 ± 1.8  46.1 ± 4.5* 
 2.0 μmol/L As2O3 18.6 ± 4.2 22.6 ± 3.9  12.8 ± 2.6  48.1 ± 4.2* 
% Cells
sub-G1G1S G2/M
HL-60/neo 
 Control  1.4 ± 1.2  33.0 ± 3.3  16.8 ± 2.5 35.5 ± 3.6  
 1.0 μmol/L As2O3 17.0 ± 2.6  40.3 ± 4.5  12.6 ± 3.5 30.3 ± 5.6  
 2.0 μmol/L As2O3 24.6 ± 3.5  39.3 ± 2.1  12.5 ± 2.7 27.1 ± 6.3  
HL-60/Bcr-abl  
 Control 1.5 ± 1.8  40.8 ± 5.6  18.1 ± 2.1 30.7 ± 2.5  
 1.0 μmol/L As2O3 12.0 ± 3.6  32.2 ± 4.1  8.1 ± 3.4 51.4 ± 4.9* 
 2.0 μmol/L As2O3 21.1 ± 5.5  20.7 ± 3.6  9.4 ± 4.7 53.2 ± 5.6* 
K562  
 Control  3.1 ± 1.9 38.2 ± 2.9  16.7 ± 1.9  36.7 ± 3.1  
 1.0 μmol/L As2O3 10.5 ± 2.5 26.1 ± 5.4  12.4 ± 1.8  46.1 ± 4.5* 
 2.0 μmol/L As2O3 18.6 ± 4.2 22.6 ± 3.9  12.8 ± 2.6  48.1 ± 4.2* 

Effects of As2O3 on the cell-cycle status and apoptosis in HL-60/neo, HL-60/Bcr-Abl, and K562 cells. Cells were incubated with the indicated concentrations of As2O3 for 7 days, stained with propidium iodide, and assayed by flow cytometry. The percentages of cells in the various phases of the cell cycle and of cells containing subdiploid DNA content (sub-G1) were determined. Data represent the mean ± SD of 3 independent experiments.

*

Values are significantly different than those observed in HL-60/neo cells treated with 1 or 2 μmol/L As2O3 for 7 days (P < .05).

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