As2O3-induced mitochondrial ▵Ψm, ROS, CD11b expression, and apoptosis
| . | % Positive Cells . | |||||||
|---|---|---|---|---|---|---|---|---|
| 24 h . | 72 h . | |||||||
| A-V/PI . | ΔΨm . | ROS . | CD11b . | A-V/PI . | ΔΨm . | ROS . | CD11b . | |
| HL-60/neo control | 1.4/0.0 | 17.8 | 3.7 | 5.8 | 3.1/1.4 | 12.0 | 8.5 | 3.9 |
| HL-60/neo 1.0 μmol/L arsenic | 5.2/2.6 | 26.9 | 4.8 | 2.1 | 11.7/4.6 | 12.3 | 12.0 | 8.8 |
| HL-60/neo 2.0 μmol/L arsenic | 11.6/4.6 | 32.9 | 10.2 | 2.6 | 18.0/6.8 | 27.9 | 54.1 | 12.6 |
| HL-60/Bcr-Abl control | 2.4/1.4 | 16.6 | 1.4 | 2.1 | 4.7/1.1 | 9.0 | 1.1 | 1.6 |
| HL-60/Bcr-Abl 1.0 μmol/L arsenic | 6.8/3.1 | 22.9 | 2.3 | 3.0 | 11.4/6.6 | 11.3 | 2.1 | 8.5 |
| HL-60/Bcr-Abl 2.0 μmol/L arsenic | 8.9/1.3 | 27.4 | 4.4 | 5.8 | 12.4/4.2 | 28.3 | 21.3 | 25.1 |
| K562 control | 2.9/1.9 | 10.7 | 3.7 | 1.4 | 3.8/1.1 | 3.7 | 3.0 | 1.1 |
| K562 1.0 μmol/L arsenic | 7.2/2.2 | 17.5 | 4.4 | 1.4 | 7.2/2.8 | 4.2 | 4.0 | 3.8 |
| K562 2.0 μmol/L arsenic | 8.8/2.9 | 19.2 | 4.4 | 2.2 | 13.2/4.4 | 9.4 | 23.6 | 20.6 |
| . | % Positive Cells . | |||||||
|---|---|---|---|---|---|---|---|---|
| 24 h . | 72 h . | |||||||
| A-V/PI . | ΔΨm . | ROS . | CD11b . | A-V/PI . | ΔΨm . | ROS . | CD11b . | |
| HL-60/neo control | 1.4/0.0 | 17.8 | 3.7 | 5.8 | 3.1/1.4 | 12.0 | 8.5 | 3.9 |
| HL-60/neo 1.0 μmol/L arsenic | 5.2/2.6 | 26.9 | 4.8 | 2.1 | 11.7/4.6 | 12.3 | 12.0 | 8.8 |
| HL-60/neo 2.0 μmol/L arsenic | 11.6/4.6 | 32.9 | 10.2 | 2.6 | 18.0/6.8 | 27.9 | 54.1 | 12.6 |
| HL-60/Bcr-Abl control | 2.4/1.4 | 16.6 | 1.4 | 2.1 | 4.7/1.1 | 9.0 | 1.1 | 1.6 |
| HL-60/Bcr-Abl 1.0 μmol/L arsenic | 6.8/3.1 | 22.9 | 2.3 | 3.0 | 11.4/6.6 | 11.3 | 2.1 | 8.5 |
| HL-60/Bcr-Abl 2.0 μmol/L arsenic | 8.9/1.3 | 27.4 | 4.4 | 5.8 | 12.4/4.2 | 28.3 | 21.3 | 25.1 |
| K562 control | 2.9/1.9 | 10.7 | 3.7 | 1.4 | 3.8/1.1 | 3.7 | 3.0 | 1.1 |
| K562 1.0 μmol/L arsenic | 7.2/2.2 | 17.5 | 4.4 | 1.4 | 7.2/2.8 | 4.2 | 4.0 | 3.8 |
| K562 2.0 μmol/L arsenic | 8.8/2.9 | 19.2 | 4.4 | 2.2 | 13.2/4.4 | 9.4 | 23.6 | 20.6 |
Various cell types were incubated with or without 1 or 2 μmol/L As2O3 for 24 or 72 hours. After this treatment, the percentage of total cells demonstrating an increase in the mitochondrial ΔΨm, ROS, CD11b expression and annexin-V or PI staining was determined by flow cytometry, as described in the text.
Data represent the mean of 2 experiments performed in duplicate.
ROS, reactive oxygen species.