PCR fragments and primers used in this study
| Fragment (size) . | Forward and Reverse Primers . | Region Bound by the Primer Pair (excluding primer annealing regions) . | |
|---|---|---|---|
| Name . | Sequence (5′ → 3′)* . | ||
| F1 (192 bp) | ABOe6F1 | TGC AGT AGG AAG GAT GTG CTC GTG | nt 259 → 374/IVS6 +1 → +27 |
| ABOi6R1 | CAG TCA CTC GAC ACT ACC TGG GTC T | ||
| F2 (279 bp) | ABOe7F2 | CCG TGT CCA CTA CTA TGT CTT CAC C | nt 460 → 693 |
| ABOe7R2 | CCG TAG AAG CTG GGG TGC AG | ||
| F3 (165 bp) | ABOe7F3 | CCT GAG GAA GCT GAG GTT CAC TGC | nt 1029 → 1144 |
| ABOi7R4 | ACA ACA GGA CGG ACA AAG GAA ACA G | ||
| Fragment (size) . | Forward and Reverse Primers . | Region Bound by the Primer Pair (excluding primer annealing regions) . | |
|---|---|---|---|
| Name . | Sequence (5′ → 3′)* . | ||
| F1 (192 bp) | ABOe6F1 | TGC AGT AGG AAG GAT GTG CTC GTG | nt 259 → 374/IVS6 +1 → +27 |
| ABOi6R1 | CAG TCA CTC GAC ACT ACC TGG GTC T | ||
| F2 (279 bp) | ABOe7F2 | CCG TGT CCA CTA CTA TGT CTT CAC C | nt 460 → 693 |
| ABOe7R2 | CCG TAG AAG CTG GGG TGC AG | ||
| F3 (165 bp) | ABOe7F3 | CCT GAG GAA GCT GAG GTT CAC TGC | nt 1029 → 1144 |
| ABOi7R4 | ACA ACA GGA CGG ACA AAG GAA ACA G | ||
Underlined bases indicate mismatches with the A1 cDNA sequence.