Experimental groups
| Exp. No. . | Sex . | Wt. (kg) . | E coli Infused (CFU/kg × 109) . | E coli Col. Count at T + 2 hr (CFU/mL × 106) . | Anti-EPCR Blocking mAb (mg/kg) . | Anti-EPCR Nonblocking mAb (mg/kg) . | Survival (hs) . |
|---|---|---|---|---|---|---|---|
| Anti-EPCR blocking mAb plus sublethal E coli | |||||||
| 4 | M | 7.1 | 6.0 | 0.4 | 5 | — | 7 |
| 5 | M | 7.3 | 5.5 | 1.0 | 5 | — | 12 |
| 7 | M | 5.7 | 4.8 | 0.8 | 5 | — | 54 |
| 10 | F | 7.7 | 5.0 | 1.5 | 5 (fab) | — | 32 |
| Average | 7.0 | 5.3 | 0.9 | 5 | — | 26.3 | |
| SE | 0.4 | 0.3 | 0.2 | 0 | — | 10.7 | |
| Anti-EPCR nonblocking mAb plus sublethal E coli | |||||||
| 6 | M | 5.9 | 5.5 | 2.3 | — | 5 | 168 |
| 9 | F | 6.4 | 4.7 | 3.0 | — | 5 (fab) | 168 |
| 11 | M | 11.1 | 6.0 | 1.0 | — | 5 | 168 |
| Average | 7.8 | 5.4 | 2.1 | — | 5 | 168 | |
| SE | 1.7 | 0.4 | 0.6 | — | 0 | 0 | |
| Saline plus sublethal E coli | |||||||
| 11294 | F | 4.3 | 5.4 | 0.4 | — | — | 24* |
| 2994 | M | 5.1 | 5.4 | 0.8 | — | — | 24* |
| 21694 | M | 5.1 | 5.8 | 0.1 | — | — | 24* |
| 31694 | M | 5.1 | 5.8 | 0.8 | — | — | 168 |
| 32394 | M | 4.8 | 4.8 | 0.6 | — | — | 168 |
| 72795 | M | 13.6 | 5.8 | 1.4 | — | — | 168 |
| 81897 | F | 11.8 | 5.8 | 0.2 | — | — | 168 |
| Average | 7.1 | 5.5 | 0.6 | — | — | 168 | |
| SE | 1.5 | 0.1 | 0.2 | — | — | 0 | |
| EPCR blocking mAb | |||||||
| 3 | F | 5.7 | — | — | 5 | — | 24* |
| 12 | F | 8.2 | — | — | 5 | — | 168 |
| Exp. No. . | Sex . | Wt. (kg) . | E coli Infused (CFU/kg × 109) . | E coli Col. Count at T + 2 hr (CFU/mL × 106) . | Anti-EPCR Blocking mAb (mg/kg) . | Anti-EPCR Nonblocking mAb (mg/kg) . | Survival (hs) . |
|---|---|---|---|---|---|---|---|
| Anti-EPCR blocking mAb plus sublethal E coli | |||||||
| 4 | M | 7.1 | 6.0 | 0.4 | 5 | — | 7 |
| 5 | M | 7.3 | 5.5 | 1.0 | 5 | — | 12 |
| 7 | M | 5.7 | 4.8 | 0.8 | 5 | — | 54 |
| 10 | F | 7.7 | 5.0 | 1.5 | 5 (fab) | — | 32 |
| Average | 7.0 | 5.3 | 0.9 | 5 | — | 26.3 | |
| SE | 0.4 | 0.3 | 0.2 | 0 | — | 10.7 | |
| Anti-EPCR nonblocking mAb plus sublethal E coli | |||||||
| 6 | M | 5.9 | 5.5 | 2.3 | — | 5 | 168 |
| 9 | F | 6.4 | 4.7 | 3.0 | — | 5 (fab) | 168 |
| 11 | M | 11.1 | 6.0 | 1.0 | — | 5 | 168 |
| Average | 7.8 | 5.4 | 2.1 | — | 5 | 168 | |
| SE | 1.7 | 0.4 | 0.6 | — | 0 | 0 | |
| Saline plus sublethal E coli | |||||||
| 11294 | F | 4.3 | 5.4 | 0.4 | — | — | 24* |
| 2994 | M | 5.1 | 5.4 | 0.8 | — | — | 24* |
| 21694 | M | 5.1 | 5.8 | 0.1 | — | — | 24* |
| 31694 | M | 5.1 | 5.8 | 0.8 | — | — | 168 |
| 32394 | M | 4.8 | 4.8 | 0.6 | — | — | 168 |
| 72795 | M | 13.6 | 5.8 | 1.4 | — | — | 168 |
| 81897 | F | 11.8 | 5.8 | 0.2 | — | — | 168 |
| Average | 7.1 | 5.5 | 0.6 | — | — | 168 | |
| SE | 1.5 | 0.1 | 0.2 | — | — | 0 | |
| EPCR blocking mAb | |||||||
| 3 | F | 5.7 | — | — | 5 | — | 24* |
| 12 | F | 8.2 | — | — | 5 | — | 168 |
EPCR = Endothelial cell protein C receptor; E coli = Escherichia coli; CFU = colony-forming units.
One animal (#8) that was infused with nonblocking EPCR mAb plus sublethal E coli went into estrus and died after 78 hs. As this animal had “positive” blood culture before infusion of E coli, and had been found to have had been in early estrus, and because estrus increases sensitivity to E coli this animal was excluded from the summary 1 above studies.