Cytokine production from OVA- or anti-CD3–stimulated splenocytes in Stat5a−/− mice and Stat5b−/− mice
| Cytokines . | Medium . | OVA . | anti-CD3 . |
|---|---|---|---|
| IL-4 (pg/mL) | |||
| Wild type | ND | 95.0 ± 37.0 | 2990.0 ± 598.3 |
| Stat5a−/− | ND | ND* | 328.0 ± 281.01-160 |
| Stat5b−/− | ND | ND* | 317.5 ± 173.51-160 |
| IL-5 (pg/mL) | |||
| Wild type | ND | 262.5 ± 68.7 | 4303.0 ± 1245.5 |
| Stat5a−/− | ND | ND* | 935.0 ± 535.51-160 |
| Stat5b−/− | ND | ND* | 931.4 ± 545.51-160 |
| IFN-γ (ng/mL) | |||
| Wild type | ND | 11.3 ± 4.8 | 401.6 ± 49.2 |
| Stat5a−/− | ND | ND* | 335.5 ± 91.4 |
| Stat5b−/− | ND | ND* | 236.5 ± 90.0* |
| Cytokines . | Medium . | OVA . | anti-CD3 . |
|---|---|---|---|
| IL-4 (pg/mL) | |||
| Wild type | ND | 95.0 ± 37.0 | 2990.0 ± 598.3 |
| Stat5a−/− | ND | ND* | 328.0 ± 281.01-160 |
| Stat5b−/− | ND | ND* | 317.5 ± 173.51-160 |
| IL-5 (pg/mL) | |||
| Wild type | ND | 262.5 ± 68.7 | 4303.0 ± 1245.5 |
| Stat5a−/− | ND | ND* | 935.0 ± 535.51-160 |
| Stat5b−/− | ND | ND* | 931.4 ± 545.51-160 |
| IFN-γ (ng/mL) | |||
| Wild type | ND | 11.3 ± 4.8 | 401.6 ± 49.2 |
| Stat5a−/− | ND | ND* | 335.5 ± 91.4 |
| Stat5b−/− | ND | ND* | 236.5 ± 90.0* |
Splenocytes from OVA-sensitized mice were stimulated with OVA (200 μg/mL) or plate-coated anti-CD3 mAb for 72 h, and the amount of IL-4, IL-5, and IFN-γ in the culture supernatant was determined by ELISA. Data are means ± SD for 5 mice in each group. The minimum significant values of these assays were 20 pg/mL of IL-4 and IL-5, and 0.1 ng/mL of IFN-γ. ND = not detectable.
P < .01,