Inhibition of GCP-2–induced migration of CXCR2-expressing cells by pertussis toxin
| . | −PTx . | +PTx . |
|---|---|---|
| Control | 1.33 ± 0.23 | 0.47 ± 0.23 |
| GCP-2 100 ng/mL | 142 ± 20.4 | 1.2 ± 0.72 |
| . | −PTx . | +PTx . |
|---|---|---|
| Control | 1.33 ± 0.23 | 0.47 ± 0.23 |
| GCP-2 100 ng/mL | 142 ± 20.4 | 1.2 ± 0.72 |
CXCR2-transfected HEK 293 cells were incubated with 100 ng/ml Pertussis toxin for 2 hours, washed and subjected to chemotaxis assays in response to 100 ng/ml GCP-2, as described in “Materials and methods.” The mean ± SD of a representative experiment of 3 performed is shown. P < .001 for migration after the treatment, vs without treatment, by pertussis toxin.