Analysis of single Hodgkin and Reed-Sternberg cells for immunoglobulin heavy and light chain gene rearrangements
| Patient . | Rearrangements of the Ig Heavy Chain Gene . | Rearrangements of the Ig Kappa Light Chain Gene . | ||||||
|---|---|---|---|---|---|---|---|---|
| Isolated HRS Cells . | HRS Cells with IgH-R . | Sequences Identical/Unrelated . | VH Family . | Isolated HRS Cells . | HRS Cells with IgL-R . | Sequences Identical/Unrelated . | VK Family . | |
| 1 | 54 | 28 | 28/0 | VH3 | 27 | 12 | 12/0 | VK2 |
| 2 | 53 | 12 | 12/0 | VH3 | 15 | 3 | 3/0 | VK2 |
| 3 | 15 | 6 | 6/0 | VH3 | 24 | 0 | 0 | — |
| 4 | 15 | 10 | 10/0 | VH3 | 17 | 5 | 5/0 | VK2 |
| 5 | 37 | 6 | 6/0 | VH1 | na | na | na | na |
| 6 | 33 | 9 | 9/0 | VH3 | 24 | 0 | 0 | — |
| 7 | 29 | 11 | 11/0 | VH3 | 16 | 6 | 6/0 | VK3 |
| 8 | 30 | 5 | 5/0 | VH3 | 14 | 3 | 3/0 | VK1 |
| 9 | 25 | 13 | 13/0 | VH3 | 10 | 2 | 2/0 | VK3 |
| 10 | 54 | 4 | 4/0 | VH3 | 16 | 4 | 4/0 | VK1 |
| 11 | 16 | 0 | 0 | — | 12 | 5 | 5/0 | VK3 |
| 12 | 50 | 0 | 0 | — | 13 | 5 | 5/0 | VK2 |
| 13 | 15 | 8 | 8/0 | VH3 | 12 | 0 | 0 | — |
| 14 | 24 | 0 | 0 | — | 18 | 0 | 0 | — |
| 15 | 48 | 12 | 12/0 | VH3 | 10 | 8 | 6(2)/0* | VK3/VK1 |
| 16 | 32 | 16 | 16/0 | † | 32 | 0 | 0 | — |
| 17 | 35 | 17 | 17/0 | VH1 | 12 | 3 | 3/0 | VK2 |
| 18 | 18 | 7 | 7/0 | VH3 | 11 | 2 | 2/0 | VK3 |
| 19 | 15 | 5 | 5/0 | VH4 | 12 | 5 | 5/0 | VK3 |
| 20 | 9 | 3 | 3/0 | VH4 | 20 | 4 | 4/0 | VK4 |
| 21 | 17 | 6 | 6/0 | VH3 | 15 | 6 | 6/0 | VK2 |
| 22 | 16 | 6 | 6/0 | VH3 | 15 | 10 | 10/0 | VK3 |
| 23 | 12 | 4 | 4/0 | VH2 | 17 | 4 | 4/0 | VK4 |
| 24 | 12 | 7 | 7/0 | VH3 | 10 | 0 | 0 | — |
| 25 | 32 | 0 | 0 | — | 10 | 9 | 9/0 | VK2 |
| Total | 696 | 195 | 195/0 | 382 | 96 | 96/0 | ||
| Patient . | Rearrangements of the Ig Heavy Chain Gene . | Rearrangements of the Ig Kappa Light Chain Gene . | ||||||
|---|---|---|---|---|---|---|---|---|
| Isolated HRS Cells . | HRS Cells with IgH-R . | Sequences Identical/Unrelated . | VH Family . | Isolated HRS Cells . | HRS Cells with IgL-R . | Sequences Identical/Unrelated . | VK Family . | |
| 1 | 54 | 28 | 28/0 | VH3 | 27 | 12 | 12/0 | VK2 |
| 2 | 53 | 12 | 12/0 | VH3 | 15 | 3 | 3/0 | VK2 |
| 3 | 15 | 6 | 6/0 | VH3 | 24 | 0 | 0 | — |
| 4 | 15 | 10 | 10/0 | VH3 | 17 | 5 | 5/0 | VK2 |
| 5 | 37 | 6 | 6/0 | VH1 | na | na | na | na |
| 6 | 33 | 9 | 9/0 | VH3 | 24 | 0 | 0 | — |
| 7 | 29 | 11 | 11/0 | VH3 | 16 | 6 | 6/0 | VK3 |
| 8 | 30 | 5 | 5/0 | VH3 | 14 | 3 | 3/0 | VK1 |
| 9 | 25 | 13 | 13/0 | VH3 | 10 | 2 | 2/0 | VK3 |
| 10 | 54 | 4 | 4/0 | VH3 | 16 | 4 | 4/0 | VK1 |
| 11 | 16 | 0 | 0 | — | 12 | 5 | 5/0 | VK3 |
| 12 | 50 | 0 | 0 | — | 13 | 5 | 5/0 | VK2 |
| 13 | 15 | 8 | 8/0 | VH3 | 12 | 0 | 0 | — |
| 14 | 24 | 0 | 0 | — | 18 | 0 | 0 | — |
| 15 | 48 | 12 | 12/0 | VH3 | 10 | 8 | 6(2)/0* | VK3/VK1 |
| 16 | 32 | 16 | 16/0 | † | 32 | 0 | 0 | — |
| 17 | 35 | 17 | 17/0 | VH1 | 12 | 3 | 3/0 | VK2 |
| 18 | 18 | 7 | 7/0 | VH3 | 11 | 2 | 2/0 | VK3 |
| 19 | 15 | 5 | 5/0 | VH4 | 12 | 5 | 5/0 | VK3 |
| 20 | 9 | 3 | 3/0 | VH4 | 20 | 4 | 4/0 | VK4 |
| 21 | 17 | 6 | 6/0 | VH3 | 15 | 6 | 6/0 | VK2 |
| 22 | 16 | 6 | 6/0 | VH3 | 15 | 10 | 10/0 | VK3 |
| 23 | 12 | 4 | 4/0 | VH2 | 17 | 4 | 4/0 | VK4 |
| 24 | 12 | 7 | 7/0 | VH3 | 10 | 0 | 0 | — |
| 25 | 32 | 0 | 0 | — | 10 | 9 | 9/0 | VK2 |
| Total | 696 | 195 | 195/0 | 382 | 96 | 96/0 | ||
Presence of two clonal IgLκ rearrangements.
A determination of the rearranged VH segment was not possible due to a 160 base pair deletion.
−No Ig rearrangement detectable; na, not analyzed.
IgH-R indicates immunoglobulin heavy chain gene rearrangement; IgLκ-R, immunoglobulin kappa light chain gene rearrangement.