Sequences and positions of primers used in the polymerase chain reaction amplification of GPIbr, GPIbβ, and GPIX genes
| Primer . | Position . | Sequence (5′ → 3′) . |
|---|---|---|
| GP Ibα 1 | 826-843 | GTGCAGTGTGACAATTCA |
| GP Ibα 2 | 1518-1501 | GTCATTTCTGGAGCTCTC |
| GP Ibα 3 | 1192-1220 | CCAAGCCCGACCACCTCAGAGCCC |
| GP Ibα 4 | 1965-1942 | AGCCCACAGGCTCTTCTCTCAAGG |
| GP Ibβ 1 | 47-72 | CTGAGCTTACTGCTCCTGCTGCTGGC |
| GP Ibβ 2 | 939-920 | GGGTTTATTCAGCACCAGAG |
| GP Ibβ 3 | 378-359 | ACGCAACGCAGGTCGCGGTA |
| GP IX 1 | 271-288 | ACCAAGGACTGCCCCAGC |
| GP IX 2 | 750-733 | ATCCAGGGCCTCTGTGGT |
| Primer . | Position . | Sequence (5′ → 3′) . |
|---|---|---|
| GP Ibα 1 | 826-843 | GTGCAGTGTGACAATTCA |
| GP Ibα 2 | 1518-1501 | GTCATTTCTGGAGCTCTC |
| GP Ibα 3 | 1192-1220 | CCAAGCCCGACCACCTCAGAGCCC |
| GP Ibα 4 | 1965-1942 | AGCCCACAGGCTCTTCTCTCAAGG |
| GP Ibβ 1 | 47-72 | CTGAGCTTACTGCTCCTGCTGCTGGC |
| GP Ibβ 2 | 939-920 | GGGTTTATTCAGCACCAGAG |
| GP Ibβ 3 | 378-359 | ACGCAACGCAGGTCGCGGTA |
| GP IX 1 | 271-288 | ACCAAGGACTGCCCCAGC |
| GP IX 2 | 750-733 | ATCCAGGGCCTCTGTGGT |
Numbering is according to published complementary DNA sequences.20,23 25